PUBLICATION
Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression
- Authors
- Wang, N., Sun, Y.H., Liu, J., Wang, Y.P., and Zhu, Z.Y.
- ID
- ZDB-PUB-070303-32
- Date
- 2007
- Source
- Development genes and evolution 217(4): 299-305 (Journal)
- Registered Authors
- Liu, Jing, Sun, Yonghua
- Keywords
- Common carp, Maternal expression, Phylogenetic analysis, Sonic hedgehog
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Carps/genetics*
- Cloning, Molecular
- DNA, Complementary/isolation & purification
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental*
- Hedgehog Proteins/chemistry
- Hedgehog Proteins/genetics*
- Hedgehog Proteins/metabolism
- Molecular Sequence Data
- Phylogeny
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Sequence Analysis, DNA
- Zebrafish
- PubMed
- 17318658 Full text @ Dev. Genes Evol.
Citation
Wang, N., Sun, Y.H., Liu, J., Wang, Y.P., and Zhu, Z.Y. (2007) Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression. Development genes and evolution. 217(4):299-305.
Abstract
Sonic hedgehog (Shh), one of important homologous members of the hedgehog (Hh) family in vertebrates, encodes a signaling molecule that is involved in short- or long-range patterning processes during embryogenesis. In zebrafish, maternal activity of Hh was found to be contributing to the formation of primary motoneurons. However, we found that all of the known Hh members were not maternally expressed in zebrafish. In the present study, full-length cDNA of common carp (Cyprinus carpio) Shh (cShh) was gained by degenerate reverse-transcription PCR (RT-PCR) and rapid amplification of cDNA ends. Sequence comparison shows that cShh coding sequence shares 93.4% identity with zebrafish Shh coding sequence, and their corresponding protein sequences have 91.9% similarity. Comparative analysis of Shh genomic sequences and Hh protein sequences from different species revealed that the genomic structures of Hh are conserved from invertebrate to vertebrate. In contrast to zebrafish Shh, cShh transcripts were detectable from one-cell stage by RT-PCR analysis. Whole mount in situ hybridization verified the maternal expression of Shh in common carp, which is, to our knowledge, the first report of that in vertebrates, suggesting that Shh might be responsible for the maternal Hh activity in common carp.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping