Identification, cDNA cloning, and mRNA localization of a zebrafish ortholog of leukemia inhibitory factor
- Abe, T., Mikekado, T., Haga, S., Kisara, Y., Watanabe, K., Kurokawa, T., and Suzuki, T.
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 147(1): 38-44 (Journal)
- Registered Authors
- Suzuki, Tohru
- MeSH Terms
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Complementary/genetics*
- Databases, Genetic
- Gene Expression Profiling
- Gene Expression Regulation
- Leukemia Inhibitory Factor/chemistry
- Leukemia Inhibitory Factor/genetics*
- Leukemia Inhibitory Factor/metabolism*
- Molecular Sequence Data
- RNA Transport*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- 17307004 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Abe, T., Mikekado, T., Haga, S., Kisara, Y., Watanabe, K., Kurokawa, T., and Suzuki, T. (2007) Identification, cDNA cloning, and mRNA localization of a zebrafish ortholog of leukemia inhibitory factor. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 147(1):38-44.
Leukemia inhibitory factor (LIF) maintains embryonic stem cells in an undifferentiated state. To establish stable cultures of zebrafish embryonic stem cells, recombinant zebrafish LIF (zLIF) is needed because the LIF sequence varies greatly between species. In the current study, we identified the zebrafish (Danio rerio) and pufferfish (Tetraodon nigroviridis) orthologs of lif from genomic databases, and we isolated a cDNA encoding zLIF. Synteny analysis and comparison of sequences identified zebrafish and Tetraodon orthologs of human LIF. The cDNA for zLIF encoded a predicted 215-amino acid protein with a putative 32-amino acid signal peptide, two disulfide bonds, and two N-linked glycosylation sites. We found that transcription of zlif starts at the hatching period during embryogenesis and is present in the brain, visceral organs, bone, and skin.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes