Differential requirement for MuSK and dystroglycan in generating patterns of neuromuscular innervation
- Lefebvre, J.L., Jing, L., Becaficco, S., Franzini-Armstrong, C., and Granato, M.
- Proceedings of the National Academy of Sciences of the United States of America 104(7): 2483-2488 (Journal)
- Registered Authors
- Granato, Michael, Jing, Lili, Lefebvre, Julie
- MeSH Terms
- Homeodomain Proteins
- Neuromuscular Junction
- RNA, Messenger
- Receptor Protein-Tyrosine Kinases/genetics
- Receptor Protein-Tyrosine Kinases/physiology*
- Signal Transduction
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- 17284594 Full text @ Proc. Natl. Acad. Sci. USA
Lefebvre, J.L., Jing, L., Becaficco, S., Franzini-Armstrong, C., and Granato, M. (2007) Differential requirement for MuSK and dystroglycan in generating patterns of neuromuscular innervation. Proceedings of the National Academy of Sciences of the United States of America. 104(7):2483-2488.
Vertebrates display diverse patterns of neuromuscular innervation, but little is known about how such diversity is generated. In mammals, neuromuscular junctions form predominantly at equatorial locations, giving rise to a focal innervation pattern along a central endplate band. In addition, vertebrate striated muscles exhibit two nonfocal neuromuscular patterns, myoseptal and distributed innervation. Although agrin-MuSK-rapsyn signaling is essential for the focal innervation pattern, it is unknown whether the same genetic program also controls synaptogenesis at nonfocal innervation sites. Here we show that one of three transcripts generated by the zebrafish unplugged locus, unplugged FL, encodes the zebrafish MuSK ortholog. We demonstrate that UnpFL/MuSK is critical for the assembly of focal synapses in zebrafish and that it cooperates with dystroglycan in the formation of nonfocal myoseptal and distributed synapses. Our results provide the first genetic evidence that neuromuscular synapse formation can occur in the absence of MuSK and that the combinatorial function of UnpFL/MuSK and dystroglycan generates diverse patterns of vertebrate neuromuscular innervation.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes