Identification of zebrafish histamine H(1), H(2) and H(3) receptors and effects of histaminergic ligands on behavior
- Peitsaro, N., Sundvik, M., Anichtchik, O.V., Kaslin, J., and Panula, P.
- Biochemical pharmacology 73(8): 1205-1214 (Journal)
- Registered Authors
- Anichtchik, Oleg, Kaslin, Jan, Panula, Pertti, Peitsaro, Nina
- G-protein-coupled receptors, Thioperamide, Tuberomamillary nucleus, Automated behavioral analysis, Danio rerio, Pyrilamine, Cimetidine, Immepip
- MeSH Terms
- Behavior, Animal/drug effects*
- Behavior, Animal/physiology
- Histamine Agonists/pharmacology*
- Histamine Antagonists/pharmacology*
- Receptors, Histamine H1/isolation & purification*
- Receptors, Histamine H2/isolation & purification*
- Receptors, Histamine H3/isolation & purification*
- 17266939 Full text @ Biochem. Pharmacol.
Peitsaro, N., Sundvik, M., Anichtchik, O.V., Kaslin, J., and Panula, P. (2007) Identification of zebrafish histamine H(1), H(2) and H(3) receptors and effects of histaminergic ligands on behavior. Biochemical pharmacology. 73(8):1205-1214.
Neuronal histamine regulates several functions in the vertebrate brain. The zebrafish brain contains a widespread histaminergic system and H(3) receptor ligand binding has been reported. In this study we provide evidence for the existence of histamine H(1), H(2) and H(3) receptor genes in zebrafish. Single copies of putative histamine H(1), H(2) and H(3) receptors were identified and cloned from the zebrafish brain. Expression analysis suggested that they are expressed in the brain and a few other tissues. Widespread distribution of zebrafish H(2) receptor binding sites was detected with [(125)I]iodoaminopotentidine in brain sections. Zebrafish larvae were exposed to 1, 10 or 100muM of the H(1) ligand pyrilamine, the H(2) ligand cimetidine and the H(3) ligands thioperamide and immepip for 5 days. Significant decreases in swimming distance were observed with the highest dose of all ligands, whereas cimetidine gave a significant decrease also with 1 and 10muM doses. These results provide the first molecular biological evidence for the presence of histamine receptors in zebrafish. These histamine receptors resemble those of higher vertebrates and they provide a useful model for pharmacological and behavioral studies for characterizing the functions of histamine in more detail.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes