PUBLICATION
A shifted repertoire of endocannabinoid genes in the zebrafish (Danio rerio)
- Authors
- McPartland, J.M., Glass, M., Matias, I., Norris, R.W., and Kilpatrick, C.W.
- ID
- ZDB-PUB-070210-39
- Date
- 2007
- Source
- Molecular genetics and genomics : MGG 277(5): 555-570 (Journal)
- Registered Authors
- Keywords
- Cannabinoid, Zebrafish genome, TRPV1, PTGS2, PPARĪ³
- MeSH Terms
-
- Chromosome Mapping/methods
- Calcium Channels
- Amidohydrolases/genetics
- Genome
- TRPV Cation Channels
- Cannabinoid Receptor Modulators/genetics*
- PPAR gamma/genetics
- Zebrafish/genetics*
- Protein Tyrosine Phosphatases
- Endocannabinoids*
- Molecular Sequence Data
- Phospholipase D/genetics
- Transient Receptor Potential Channels/genetics
- Lipoprotein Lipase/genetics
- Animals
- Humans
- Cyclooxygenase 2/genetics
- Phylogeny
- Nerve Tissue Proteins
- Zebrafish Proteins/genetics*
- Receptor, Cannabinoid, CB1/genetics
- Algorithms
- Receptor, Cannabinoid, CB2/genetics
- PPAR alpha/genetics
- Amino Acid Sequence
- Protein Tyrosine Phosphatase, Non-Receptor Type 22
- PubMed
- 17256142 Full text @ Mol. Genet. Genomics
Citation
McPartland, J.M., Glass, M., Matias, I., Norris, R.W., and Kilpatrick, C.W. (2007) A shifted repertoire of endocannabinoid genes in the zebrafish (Danio rerio). Molecular genetics and genomics : MGG. 277(5):555-570.
Abstract
The zebrafish has served as a model organism for developmental biology. Sequencing its genome has expanded zebrafish research into physiology and drug-development testing. Several cannabinoid pharmaceuticals are in development, but expression of endocannabinoid receptors and enzymes remains unknown in this species. We conducted a bioinformatics analysis of the zebrafish genome using 17 human endocannabinoid genes as a reference set. Putative zebrafish orthologs were identified in filtered BLAST searches as reciprocal best hits. Orthology was confirmed by three in silico methods: phylogenetic testing, synteny analysis, and functional mapping. Zebrafish expressed orthologs of cannabinoid receptor 1, transient receptor potential channel vanilloid receptor 4, GPR55 receptor, fatty acid amide hydrolase 1, monoacylglycerol lipase, NAPE-selective phospholipase D, abhydrolase domain-containing protein 4, and diacylglycerol lipase alpha and beta; and paired paralogs of cannabinoid receptor 2, fatty acid amide hydrolase 2, peroxisome proliferator-activated receptor alpha, prostaglandin-endoperoxide synthase 2, and transient receptor potential cation channel subtype A1. Functional mapping suggested the orthologs of transient receptor potential vanilloid receptor 1 and peroxisome proliferator-activated receptor gamma lack specific amino acids critical for cannabinoid ligand binding. No orthologs of N-acylethanolamine acid amidase or protein tyrosine phosphatase, non-receptor type 22 were identified. In conclusion, the zebrafish genome expresses a shifted repertoire of endocannabinoid genes. In vitro analyses are warranted before using zebrafish for cannabinoid development testing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping