PUBLICATION

The zebrafish orphan nuclear receptor genes nr2e1 and nr2e3 are expressed in developing eye and forebrain

Authors
Kitambi, S.S., and Hauptmann, G.
ID
ZDB-PUB-061227-5
Date
2007
Source
Gene expression patterns : GEP   7(4): 521-528 (Journal)
Registered Authors
Hauptmann, Giselbert, Kitambi, Satish Srinivas
Keywords
Tailless, Tll, Pnr, Photoreceptor, Eye, Retina, Epiphysis, Pineal, Forebrain, Zebrafish, Danio rerio, Orphan nuclear receptor
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Embryo, Nonmammalian/metabolism
  • Eye/embryology*
  • Eye/metabolism
  • Gene Expression*
  • Molecular Sequence Data
  • Phylogeny
  • Prosencephalon/embryology*
  • Prosencephalon/metabolism
  • Receptors, Cytoplasmic and Nuclear/genetics*
  • Sequence Alignment
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
PubMed
17127102 Full text @ Gene Expr. Patterns
Abstract
Mammalian Nr2e1 (Tailless, Mtll or Tlx) and Nr2e3 (photoreceptor-specific nuclear receptor, Pnr) are highly related orphan nuclear receptors, that are expressed in eye and forebrain-derived structures. In this study, we analyzed the developmental expression patterns of zebrafish nr2e1 and nr2e3. RT-PCR analysis showed that nr2e1 and nr2e3 are both expressed during embryonic and post-embryonic development. To examine the spatial distribution of nr2e1 and nr2e3 during development whole-mount in situ hybridization was performed. At tailbud stage, initial nr2e1 expression was localized to the rostral brain rudiment anterior to pax2.1 and eng2 expression at the prospective midbrain-hindbrain boundary. During subsequent stages, nr2e1 became widely expressed in fore- and midbrain primordia, eye and olfactory placodes. At 24hpf, strong nr2e1 expression was detected in telencephalon, hypothalamus, dorsal thalamus, pretectum, midbrain tectum, and retina. At 2dpf, the initially widespread nr2e1 expression became more restricted to distinct regions within the fore- and midbrain and to the retinal ciliary margin, the germinal zone which gives rise to retina and presumptive iris. Expression of nr2e3 was exclusively found in the developing retina and epiphysis. In both structures, nr2e3 expression was found in photoreceptor cells. The developmental expression profile of zebrafish nr2e1 and nr2e3 is consistent with evolutionary conserved functions in eye and rostral brain structures.
Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping