ZFIN ID: ZDB-PUB-061226-3
Genetic and Transcriptome Characterization of Model Zebrafish Cell Lines
He, S., Salas-Vidal, E., Rueb, S., Krens, S.F.G., Meijer, A.H., Snaar-Jagalska, B.E., and Spaink, H.P.
Date: 2006
Source: Zebrafish   3(4): 441-453 (Journal)
Registered Authors: He, Shuning, Krens, S. F. Gabby, Meijer, Annemarie H., Rueb, Saskia, Salas-Vidal, Enrique, Snaar-Jagalska, Ewa B., Spaink, Herman P.
Keywords: none
MeSH Terms: none
PubMed: none
Compared with the increasing use of zebrafish as a model organism in many laboratories, zebrafish cell lines are still unexploited and limited in application, partly due to their unknown genetic and physiological properties. We characterize two zebrafish embryonic fibroblast cell lines, ZF4 and PAC2. We demonstrate the genetic stability of these two zebrafish cell lines and achieved genetic manipulation by either lipid-mediated transfection or an electroporation- based nucleofection method. Data from zebrafish chip analysis (Affymetrix) demonstrate unique characteristics of these two cell lines in gene expression levels, showing that different zebrafish cell lines can be classified by their transcriptome profile. Their transcriptional responses to serum growth factor exposure suggest that zebrafish fibroblast cell lines may be used to study processes related to wound-healing or cancer.