ZFIN ID: ZDB-PUB-061222-5
The NV gene of snakehead rhabdovirus (SHRV) is not required for pathogenesis, and a heterologous glycoprotein can be incorporated into the SHRV envelope
Alonso, M., Kim, C.H., Johnson, M.C., Pressley, M., and Leong, J.A.
Date: 2004
Source: Journal of virology   78(11): 5875-5882 (Journal)
Registered Authors: Johnson, Marc, Kim, Carol H., Leong, Jo-Ann
Keywords: none
MeSH Terms:
  • Animals
  • Fish Diseases/virology*
  • Fishes
  • Genes, Viral/physiology*
  • Glycoproteins/physiology*
  • Humans
  • Infectious hematopoietic necrosis virus/genetics
  • Open Reading Frames
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rhabdoviridae/genetics*
  • Rhabdoviridae/pathogenicity
  • Viral Proteins/genetics*
  • Viral Proteins/physiology*
  • Virus Assembly
  • Virus Replication
  • Zebrafish/virology
PubMed: 15140985 Full text @ J. Virol.
Snakehead rhabdovirus (SHRV) affects warm-water fish in Southeast Asia and belongs to the genus Novirhabdovirus by virtue of its "nonvirion" (NV) gene. To examine the function of the NV gene, we used a recently developed reverse genetic system to produce a viable recombinant SHRV carrying an NV gene deletion. The recombinant virus was produced at the same rate and same final concentrations as the wild-type virus in cultured fish cells in spite of the NV gene deletion. The role of the NV protein in fish pathogenesis was also investigated. Zebra fish (Danio rerio) were infected with the NV deletion mutant or with a recombinant virus containing a copy of the SHRV genome, and similar mortality rates as well as final mortalities were recorded, suggesting no apparent role for the NV protein in fish pathogenesis. Interestingly, the unsuccessful rescue of fully viable recombinants with genomes containing deletions in the G/NV gene junction suggested a role for the gene junction in virus transcription and replication. Finally, we demonstrated that the SHRV glycoprotein can be replaced by the glycoprotein of infectious hematopoietic necrosis virus (IHNV) or by a hybrid protein composed of SHRV and IHNV sequences.