PUBLICATION
Laser-induced thrombosis in zebrafish larvae: a novel genetic screening method for thrombosis
- Authors
- Jagadeeswaran, P., Paris, R., and Rao, P.
- ID
- ZDB-PUB-061108-25
- Date
- 2006
- Source
- Methods in molecular medicine 129: 187-196 (Chapter)
- Registered Authors
- Jagadeeswaran, Pudur
- Keywords
- none
- MeSH Terms
-
- Animals
- Blood Coagulation Disorders/diagnosis*
- Breeding
- Hemostasis/physiology*
- Lasers
- Models, Animal*
- Sepharose
- Thrombosis*
- Zebrafish/genetics
- Zebrafish/physiology*
- PubMed
- 17085812 Full text @ Methods Mol. Med.
Citation
Jagadeeswaran, P., Paris, R., and Rao, P. (2006) Laser-induced thrombosis in zebrafish larvae: a novel genetic screening method for thrombosis. Methods in molecular medicine. 129:187-196.
Abstract
Classical genetic approaches to study hemostasis and thrombosis have not been available until our recent introduction of the teleost, Danio rerio (the zebrafish), as an effective genetic model for in vivo coagulation assays. The genetic screen for this model is carried out using the genome saturation mutagenesis approach. The resulting mutants are screened for hemostatic or thrombotic defects. We developed a global physiological screening method for thrombosis by utilizing a laser to induce thrombosis in a specifically targeted area of the major artery and vein. Using this assay, we have screened many fish for abnormal hemostasis, and have isolated a number of mutants with abnormal coagulation parameters. These mutants can be grown, bred, and further evaluated for the genetic etiology of their abnormal hemostatic pathways.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping