Migration of zebrafish primordial germ cells: a role for Myosin contraction and cytoplasmic flow
- Blaser, H., Reichman-Fried, M., Castanon, I., Dumstrei, K., Marlow, F.L., Kawakami, K., Solnica-Krezel, L., Heisenberg, C.P., and Raz, E.
- Developmental Cell 11(5): 613-627 (Journal)
- Registered Authors
- Blaser, Heiko, Dumstrei, Karin, Heisenberg, Carl-Philipp, Kawakami, Koichi, Marlow, Florence, Raz, Erez, Reichman-Fried, Michal, Solnica-Krezel, Lilianna
- MeSH Terms
- Cell Membrane/physiology
- Cell Polarity
- Chemokine CXCL12
- Chemokines, CXC/physiology
- Germ Cells/physiology*
- Receptors, CXCR4/physiology
- Zebrafish Proteins/physiology*
- 17084355 Full text @ Dev. Cell
Blaser, H., Reichman-Fried, M., Castanon, I., Dumstrei, K., Marlow, F.L., Kawakami, K., Solnica-Krezel, L., Heisenberg, C.P., and Raz, E. (2006) Migration of zebrafish primordial germ cells: a role for Myosin contraction and cytoplasmic flow. Developmental Cell. 11(5):613-627.
The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes