PUBLICATION

Identification of a Promoter Element within the Zebrafish colXalpha1 Gene Responsive to Runx2 Isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2

Authors
Simoes, B., Conceicao, N., Viegas, C.S., Pinto, J.P., Gavaia, P.J., Hurst, L.D., Kelsh, R.N., and Cancela, M.L.
ID
ZDB-PUB-061020-35
Date
2006
Source
Calcified tissue international   79(4): 230-244 (Journal)
Registered Authors
Kelsh, Robert
Keywords
Runx2, colXalpha1, Evolutionary conservation, Promoter, Transcriptional regulation
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bone Development/genetics*
  • Cloning, Molecular
  • Collagen Type X/genetics*
  • Core Binding Factor Alpha 1 Subunit/metabolism*
  • Gene Expression
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • Humans
  • In Situ Hybridization
  • Molecular Sequence Data
  • Phylogeny
  • Promoter Regions, Genetic
  • Protein Isoforms/metabolism*
  • RNA, Messenger/analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology
  • Transcriptional Activation
  • Zebrafish/genetics*
PubMed
17033725 Full text @ Calcif. Tissue Int.
Abstract
Type X collagen is a short chain collagen specifically expressed by hypertrophic chondrocytes during endochondral ossification. We report here the functional analysis of the zebrafish (Danio rerio) collagen Xalpha1 gene (colXalpha1) promoter with the identification of a region responsive to two isoforms of the runt domain transcription factor runx2. Furthermore, we provide evidence for the presence of dual promoter usage in zebrafish, a finding that should be important to further understanding of the regulation of its restricted tissue distribution and spatial-temporal expression during early development. The zebrafish colXalpha1 gene structure is comparable to that recently identified by comparative genomics in takifugu and shows homology with corresponding mammalian genes, indicating that its general architecture has been maintained throughout vertebrate evolution. Our data suggest that, as in mammals, runx2 plays a role in the development of the osteogenic lineage, supporting zebrafish as a model for studies of bone and cartilage development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes