PUBLICATION
Diversin regulates heart formation and gastrulation movements in development
- Authors
- Moeller, H., Jenny, A., Schaeffer, H.J., Schwarz-Romond, T., Mlodzik, M., Hammerschmidt, M., and Birchmeier, W.
- ID
- ZDB-PUB-061020-33
- Date
- 2006
- Source
- Proceedings of the National Academy of Sciences of the United States of America 103(43): 15900-15905 (Journal)
- Registered Authors
- Hammerschmidt, Matthias
- Keywords
- none
- MeSH Terms
-
- Drosophila melanogaster/embryology
- Drosophila melanogaster/genetics
- Drosophila melanogaster/metabolism
- Humans
- Signal Transduction
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Ankyrin Repeat
- JNK Mitogen-Activated Protein Kinases/metabolism
- Animals
- Cell Line
- Gene Expression Regulation, Developmental
- Gastrointestinal Tract/embryology*
- Gastrointestinal Tract/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Protein Binding
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism*
- Wnt Proteins
- Heart/embryology*
- Myocardium/metabolism*
- PubMed
- 17032765 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Moeller, H., Jenny, A., Schaeffer, H.J., Schwarz-Romond, T., Mlodzik, M., Hammerschmidt, M., and Birchmeier, W. (2006) Diversin regulates heart formation and gastrulation movements in development. Proceedings of the National Academy of Sciences of the United States of America. 103(43):15900-15905.
Abstract
Canonical and noncanonical Wnt signaling regulate crucial events in the development of vertebrates and invertebrates. In this work we show that vertebrate Diversin, a potential orthologue of Drosophila Diego, controls fusion of heart precursors and gastrulation movements in zebrafish embryogenesis. These events are regulated by noncanonical Wnt signaling, which is independent of beta-catenin. We found that Diversin directly interacts with Dishevelled and that this interaction is necessary and sufficient to mediate signals of the noncanonical Wnt pathway to downstream effectors like Rho family GTPases and Jun N-terminal kinase. The ankyrin repeats of Diversin are required for the interaction with Dishevelled, for the activation of noncanonical Wnt signaling, and for the biological responses. The mutation K446M in the DEP domain of vertebrate Dishevelled, which mimics a classical Drosophila loss of function mutation, prevents functional interaction with Diversin's ankyrin repeats. Diversin also affects planar cell polarity in Drosophila, which is controlled by the noncanonical Wnt signaling pathway. Our data thus demonstrate that Diversin and Dishevelled function together in a mutually dependent fashion in zebrafish gastrulation and organ formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping