PUBLICATION

Resolution of inflammation by retrograde chemotaxis of neutrophils in transgenic zebrafish

Authors
Mathias, J.R., Perrin, B.J., Liu, T.X., Kanki, J., Look, A.T., and Huttenlocher, A.
ID
ZDB-PUB-060921-6
Date
2006
Source
Journal of Leukocyte Biology   80(6): 1281-1288 (Journal)
Registered Authors
Huttenlocher, Anna, Kanki, John, Liu, Ting Xi, Look, A. Thomas, Mathias, Jonathan
Keywords
GFP, leukocyte
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Chemotaxis/genetics
  • Chemotaxis/immunology*
  • Genetic Markers/immunology
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/immunology
  • Inflammation/genetics
  • Inflammation/immunology
  • Inflammation/pathology
  • Microscopy, Fluorescence/methods
  • Microscopy, Video/methods
  • Neutrophil Infiltration/genetics
  • Neutrophil Infiltration/immunology*
  • Neutrophils/immunology*
  • Neutrophils/pathology
  • Peroxidase/genetics
  • Peroxidase/immunology
  • Promoter Regions, Genetic/genetics
  • Promoter Regions, Genetic/immunology
  • Wounds and Injuries/genetics
  • Wounds and Injuries/immunology*
  • Wounds and Injuries/pathology
  • Zebrafish/genetics
  • Zebrafish/immunology*
PubMed
16963624 Full text @ J. Leukoc. Biol.
Abstract
Neutrophil chemotaxis to sites of inflammation is a critical process during normal immune responses to tissue injury and infection and pathological immune responses leading to chronic inflammation. Although progress has been made in understanding the mechanisms that promote neutrophil recruitment to inflamed tissue, the mechanisms that regulate the resolution phase of the inflammatory response have remained relatively elusive. To define the mechanisms that regulate neutrophil-mediated inflammation in vivo, we have developed a novel transgenic zebrafish in which the neutrophils express GFP under control of the myeloperoxidase promoter (zMPO:GFP). Tissue injury induces a robust, inflammatory response, which is characterized by the rapid chemotaxis of neutrophils to the wound site. In vivo time-lapse imaging shows that neutrophils subsequently display directed retrograde chemotaxis back toward the vasculature. These findings implicate retrograde chemotaxis as a novel mechanism that regulates the resolution phase of the inflammatory response. The zMPO:GFP zebrafish provides unique insight into the mechanisms of neutrophil-mediated inflammation and thereby offers opportunities to identify new regulators of the inflammatory response in vivo.
Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping