ZFIN ID: ZDB-PUB-060921-15
Semaphorin 3d promotes cell proliferation and neural crest cell development downstream of TCF in the zebrafish hindbrain
Berndt, J.D., and Halloran, M.C.
Date: 2006
Source: Development (Cambridge, England)   133(20): 3983-3992 (Journal)
Registered Authors: Berndt, Jason, Halloran, Mary
Keywords: Neural crest, Zebrafish, Semaphorin, TCF, Cell cycle, Cyclin
MeSH Terms:
  • Animals
  • Body Patterning
  • Cell Movement/genetics
  • Cell Proliferation
  • Cyclins/genetics
  • Gene Expression
  • Mutation
  • Nerve Growth Factors/antagonists & inhibitors
  • Nerve Growth Factors/genetics
  • Nerve Growth Factors/physiology*
  • Neural Crest/chemistry
  • Neural Crest/cytology
  • Neural Crest/growth & development*
  • Pluripotent Stem Cells/chemistry
  • Pluripotent Stem Cells/cytology
  • Pluripotent Stem Cells/physiology*
  • RNA, Messenger/analysis
  • RNA, Messenger/metabolism
  • Rhombencephalon/chemistry
  • Rhombencephalon/cytology
  • Rhombencephalon/growth & development*
  • Semaphorins/antagonists & inhibitors
  • Semaphorins/genetics
  • Semaphorins/physiology*
  • Wnt Proteins/metabolism
  • Zebrafish/growth & development*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed: 16971468 Full text @ Development
Neural crest cells (NCCs) are pluripotent migratory cells that are crucial to the development of the peripheral nervous system, pigment cells and craniofacial cartilage and bone. NCCs are specified within the dorsal ectoderm and undergo an epithelial to mesenchymal transition (EMT) in order to migrate to target destinations where they differentiate. Here we report a role for a member of the semaphorin family of cell guidance molecules in NCC development. Morpholino-mediated knockdown of Sema3d inhibits the proliferation of hindbrain neuroepithelial cells. In addition, Sema3d knockdown reduces markers of migratory NCCs and disrupts NCC-derived tissues. Similarly, expression of a dominant-repressor form of TCF (DeltaTCF) reduces hindbrain cell proliferation and leads to a disruption of migratory NCC markers. Moreover, expression of DeltaTCF downregulates sema3d RNA expression. Finally, Sema3d overexpression rescues reduced proliferation caused by DeltaTCF expression, suggesting that Sema3d lies downstream of Wnt/TCF signaling in the molecular pathway thought to control cell cycle in NCC precursors.