PUBLICATION

Structure of the catfish IGH locus: analysis of the region including the single functional IGHM gene

Authors
Bengten, E., Quiniou, S., Hikima, J., Waldbieser, G., Warr, G.W., Miller, N.W., and Wilson, M.
ID
ZDB-PUB-060906-14
Date
2006
Source
Immunogenetics   58(10): 831-844 (Journal)
Registered Authors
Keywords
Immunoglobulin heavy chain locus, Catfish, Bacterial artificial chromosome
MeSH Terms
  • Gene Dosage
  • Base Sequence
  • Ictaluridae/genetics*
  • Ictaluridae/immunology*
  • Molecular Sequence Data
  • Genes, Immunoglobulin Heavy Chain*
  • Amino Acid Sequence
  • Immunoglobulin Variable Region/genetics
  • Animals
  • Sequence Alignment
  • Immunoglobulin mu-Chains/genetics*
  • Pseudogenes
  • Chromosomes, Artificial, Bacterial
  • Immunoglobulin Joining Region/genetics
  • Phylogeny
(all 15)
PubMed
16941126 Full text @ Immunogenetics
Abstract
The catfish IGH locus is large ( approximately 1 Mb) and complex, having undergone multiple internal duplications and transpositions. To define the structure of the locus that contains the single expressed IGHM gene, two overlapping bacterial-artificial-chromosome (BAC) clones spanning the most 3' end of the channel catfish immunoglobulin heavy (IGH) chain locus have been completely sequenced. The analyses created a contig of 257,153 bp containing 55 VH, 6 D, 12 JH genes and the IGH constant region genes encoding the functional secreted and membrane forms of IgM and the membrane form of IgD. This analysis revealed three major features. First, no C-region genes were found aside from the previously described IGHM1 and IGHD1, with the latter gene being the most 3' C-region gene of the catfish IGH locus. There was no evidence in the region sequenced for genes that could encode an Ig class similar to the IgZ/IgT described in zebrafish, trout and pufferfish. Second, there are a high number of VH pseudogenes, 28 out of 55 (51%). In contrast, the entire zebrafish IGH locus has 40 functional VH genes and eight pseudogenes (17%). Third, an internal duplication of a 52.4-kb block of VH genes has occurred. These observations suggest that the IGH locus of teleost fish varies significantly from species to species in the diversity of C-region genes as well as the numbers of genes encoding V regions.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Bengten, E., Quiniou, S., Hikima, J., Waldbieser, G., Warr, G.W., Miller, N.W., and Wilson, M. (2006) Structure of the catfish IGH locus: analysis of the region including the single functional IGHM gene. Immunogenetics. 58(10):831-844.