ZFIN ID: ZDB-PUB-060816-14
A rapid, high content, in vivo model of glucocorticoid-induced osteoporosis
Barrett, R., Chappell, C., Quick, M., and Fleming, A.
Date: 2006
Source: Biotechnology Journal   1(6): 651-655 (Journal)
Registered Authors: Fleming, Angeleen
Keywords: Glucocorticoid, Osteoporosis, Zebrafish, Animal model, High-throughput
MeSH Terms:
  • Animals
  • Calcification, Physiologic/drug effects*
  • Diphosphonates/administration & dosage*
  • Disease Models, Animal*
  • Glucocorticoids*
  • Osteoporosis/chemically induced*
  • Osteoporosis/drug therapy*
  • Osteoporosis/pathology
  • Treatment Outcome
  • Zebrafish*
PubMed: 16892313 Full text @ Biotechnol. J.
Glucocorticoid-induced osteoporosis (GIOP) is a major clinical problem given the widespread use of steroids and limited efficacy of biphosphonates. Existing animal models of GIOP are both slow and expensive. Hence, there is a need both for adjunctive modelling systems, as well as more efficacious therapies for the treatment of GIOP. We have addressed this issue through the creation of a zebrafish model of GIOP, which can be used for 96-well plate in vivo screening with an assay time of 5 days. The model demonstrates key similarities to human GIOP including a partial response to bisphosphonates. The ability to extract detailed pharmacological data, including concentration-response analyses, enables the screening and ranking of candidate therapeutic compounds. In addition, the zebrafish model is highly relevant for pathway dissection through genetic knockdown and overexpression studies.