Cloning and pattern of expression of the shiro-uo vasa gene during embryogenesis and its roles in PGC development

Miyake, A., Saito, T., Kashiwagi, N., Ando, D., Yamamoto, A., Suzuki, T., Nakatsuji, N., and Nakatsuji, T.
The International journal of developmental biology   50(7): 619-625 (Journal)
Registered Authors
Suzuki, Tohru
Shiro-uo, vasa, primordial germ cell (PGC), mRNA localization, teleost
MeSH Terms
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Blastomeres/cytology
  • Blastomeres/ultrastructure
  • Blastula/cytology
  • Blastula/ultrastructure
  • Cleavage Stage, Ovum
  • Cloning, Molecular*
  • Conserved Sequence
  • DNA, Complementary
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/ultrastructure
  • Fishes/embryology*
  • Fishes/genetics
  • Fishes/metabolism
  • Gene Expression Regulation, Developmental*
  • Germ Cells/growth & development*
  • In Situ Hybridization
  • Molecular Sequence Data
  • Phylogeny
  • RNA Helicases/genetics*
  • RNA, Messenger/metabolism
  • Sequence Homology, Amino Acid
16892175 Full text @ Int. J. Dev. Biol.
The vasa genes are expressed in the germ cell lineage in many organisms, but their expression patterns show large variations. Recent studies suggest that vasa transcripts are involved in germ cell lineage development. In this paper, we isolated the vasa cDNA clone from a teleost, shiro-uo, Leucopsarion petersii and examined its expression pattern during embryogenesis. Then, we examined the functional significance of vasa mRNA during the formation of primordial germ cells (PGCs). The amino acid sequence of shiro-uo VASA is 61.1% identical to that of zebrafish. In whole-mount in situ hybridization, vasa transcripts appeared at the 4- and 8-cell stages as four spots at both ends of two cleavage planes between the lower tier of blastomeres and the yolk cell mass. At the 16-cell stage, eight spots were observed. After the blastula stage, shiro-uo vasa transcripts showed similar localization as in the zeblafish. Ultrastructural analysis of 4-cell stage embryos revealed the presence of a subcellular organelle that resembled 'nuage' in the germ cell lineage observed in the embryos of various organisms. We carried out micromanipulation of 4- or 8-cell stage embryos to remove the vasa mRNA-containig spots and then measured the number of the vasa-expressing PGCs in the genital ridge of the manipulated embryos. The numbers decreased when all of the four spots were removed, indicating that the vasa-containing spots at early cleavage stages have important functions in the development of PGCs.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes