PUBLICATION

Lhx5 promotes forebrain development and activates transcription of secreted Wnt antagonists

Authors
Peng, G., and Westerfield, M.
ID
ZDB-PUB-060724-25
Date
2006
Source
Development (Cambridge, England)   133(16): 3191-3200 (Journal)
Registered Authors
Peng, Gang, Westerfield, Monte
Keywords
Axin, Forebrain, Lhx5, LIM-homeobox domain factor, masterblind, Secreted Wnt antagonist, Sfrp, Wnt signaling, Zebrafish
MeSH Terms
  • Animals
  • Axin Protein
  • Gene Expression Regulation, Developmental*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology*
  • Intercellular Signaling Peptides and Proteins/genetics*
  • LIM-Homeodomain Proteins
  • Prosencephalon/embryology*
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Trans-Activators/genetics
  • Trans-Activators/physiology*
  • Transcription Factors
  • Transcription, Genetic
  • Wnt Proteins/antagonists & inhibitors*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
PubMed
16854974 Full text @ Development
Abstract
In vertebrate embryos, induction and patterning of the forebrain require the local inhibition of caudalizing signals, such as Wnts, emanating from the mesendoderm and caudal brain. Here, we report that Lhx5, expressed in the rostral neuroectoderm, regulates the local inhibition of Wnts. Activation of Lhx5 expands forebrain structures, whereas inhibition of Lhx5 function compromises forebrain development in zebrafish embryos. Lhx5 can rescue forebrain deficiencies caused by excess Wnt activity, and inhibition of Lhx5 function results in ectopic activation of Wnt signaling. Lhx5 regulates the expression of two secreted Frizzled-related Wnt antagonists, Sfrp1a and Sfrp5. These Sfrps can reduce the ectopic activation of Wnt signaling and rescue the forebrain deficiencies caused by inhibition of Lhx5 function. Our results demonstrate that Lhx5 is a required factor that promotes forebrain development and inhibits Wnt signaling by activating the transcription of secreted Wnt antagonists.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes