PUBLICATION

Modified bacterial artificial chromosomes for zebrafish transgenesis

Authors
Yang, Z., Jiang, H., Chachainasakul, T., Gong, S., Yang, X.W., Heintz, N., and Lin, S.
ID
ZDB-PUB-060719-9
Date
2006
Source
Methods (San Diego, Calif.)   39(3): 183-188 (Journal)
Registered Authors
Jiang, Hong, Lin, Shuo, Yang, Zhongan
Keywords
Transgenic zebrafish, GFP, RFP, Modification, Zebrafish BAC and PAC
MeSH Terms
  • Animals
  • Animals, Genetically Modified/genetics*
  • Chromosomes, Artificial, Bacterial/genetics*
  • Embryo, Nonmammalian
  • Escherichia coli/genetics
  • Gene Expression Regulation
  • Genes, Bacterial
  • Genes, Reporter
  • Genetic Engineering/methods*
  • Green Fluorescent Proteins/analysis
  • Luminescent Proteins/analysis
  • Plasmids/genetics
  • Rec A Recombinases/genetics
  • Recombinant Fusion Proteins/analysis
  • Recombination, Genetic
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed
16828309 Full text @ Methods
Abstract
Transgenesis using bacterial artificial chromosomes (BAC) offers greater fidelity in directing desirable expression of foreign genes. Application of this technology in the optically transparent zebrafish with fluorescent protein reporters enables unparalleled visual analysis of regulation of gene expression in a living organism. Here we describe a streamlined procedure of direct selecting multiple BAC clones based on public sequence databases followed by rapid modification with GFP or RFP for transgenic analysis in zebrafish. Experimental procedures for BAC DNA preparation, microinjection of zebrafish embryos and screening of transgenic zebrafish carrying GFP/RFP modified BAC clones are detailed.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping