PUBLICATION
C terminus of RGS-GAIP-interacting protein conveys neuropilin-1-mediated signaling during angiogenesis
- Authors
- Wang, L., Mukhopadhyay, D., and Xu, X.
- ID
- ZDB-PUB-060616-33
- Date
- 2006
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20(9): 1513-1515 (Journal)
- Registered Authors
- Xu, Xiaolei
- Keywords
- none
- MeSH Terms
-
- Animals
- Cells, Cultured
- Conserved Sequence
- Endothelium, Vascular/drug effects
- Endothelium, Vascular/physiology*
- Humans
- Morpholines/pharmacology
- Neovascularization, Physiologic*
- Neuropilin-1/physiology*
- RGS Proteins/genetics
- RGS Proteins/physiology*
- RNA Interference
- RNA Splicing
- Signal Transduction/physiology*
- Umbilical Veins
- Zebrafish
- Zebrafish Proteins/physiology
- PubMed
- 16754745 Full text @ FASEB J.
Citation
Wang, L., Mukhopadhyay, D., and Xu, X. (2006) C terminus of RGS-GAIP-interacting protein conveys neuropilin-1-mediated signaling during angiogenesis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 20(9):1513-1515.
Abstract
Initially, it was thought that there was no intracellular signaling mediated by NRP-1 alone in response to its ligands. However, the emerging data from our group as well as others suggest that the signaling through NRP-1 actually promotes angiogenesis and is mediated through its C-terminal domain and downstream molecules such as phosphoinositide 3-kinase. Hence, understanding the signal transduction pathways mediated by NRP-1 and identification of its downstream molecules are of importance. By using both in vivo zebrafish model and in vitro tissue culture system, we have shown that the C-terminal three amino acids of NRP-1 (SEA-COOH) are required for NRP-1-mediated angiogenesis. Furthermore, knocking down of RGS-GAIP-interacting protein C terminus (GIPC) in zebrafish, which is associated with C-terminal domain of NRP-1, exhibits similar vasculature phenotypes to those from NRP-1 null. Specific and effective silencing of GIPC in vascular endothelium results in inhibition of NRP-1-mediated migration. In both cases as described, PDZ domain of GIPC is responsible for its function. Taken together, our data suggest a novel role of GIPC in angiogenesis and vessel formation and also support our hypothesis that NRP-1 can facilitate downstream signaling to promote angiogenesis through GIPC.--Wang, L., Mukhopadhyay, D., Xu, X. C terminus of RGS-GAIP-interacting protein conveys neuropilin-1-mediated signaling during angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping