PUBLICATION
Zebrafish and giant danio as models for muscle growth: Determinate versus indeterminate growth as determined by morphometric analysis
- Authors
- Biga, P.R., and Goetz, F.W.
- ID
- ZDB-PUB-060616-19
- Date
- 2006
- Source
- American journal of physiology. Regulatory, integrative and comparative physiology 291(5): R1327-R1337 (Journal)
- Registered Authors
- Goetz, Frederick W.
- Keywords
- hypertrophy, hyperplasia, growth hormone, model organisms
- MeSH Terms
-
- Animals
- Cyprinidae/growth & development*
- Cyprinidae/physiology
- Female
- Growth/drug effects
- Growth/physiology
- Growth Hormone/pharmacology
- Hyperplasia/pathology
- Male
- Models, Biological*
- Muscle, Skeletal/growth & development*
- Muscle, Skeletal/pathology
- Muscle, Skeletal/physiology
- Zebrafish/growth & development*
- Zebrafish/physiology
- PubMed
- 16741137 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
Citation
Biga, P.R., and Goetz, F.W. (2006) Zebrafish and giant danio as models for muscle growth: Determinate versus indeterminate growth as determined by morphometric analysis. American journal of physiology. Regulatory, integrative and comparative physiology. 291(5):R1327-R1337.
Abstract
The zebrafish has become an important genetic model, but their small size makes them impractical for traditional physiological studies. In contrast, the closely-related giant danio is larger and can be utilized for physiological studies that can also make use of the extensive zebrafish genomic resources. In addition, the giant danio and zebrafish appear to exhibit different growth types, indicating the potential for developing a comparative muscle growth model system. Therefore, the current study was conducted to compare and characterize the muscle growth pattern of zebrafish and giant danio. Morphometric analyses demonstrated that giant danio exhibit an increased growth rate compared to zebrafish, starting as early as 2 weeks post-hatch. Total myotome area, mean fiber area, and total fiber number all exhibited positive correlations with larvae length in giant danio, but not in zebrafish. Morphometric analysis of giant danio and zebrafish larvae demonstrated faster, more efficient growth in giant danio larvae. Similar to larger teleosts, adult giant danio exhibited increased growth rates and muscle hyperplasia in response to growth hormone, suggesting that giant danio exhibit indeterminate growth. In contrast, adult zebrafish do not exhibit mosaic hyperplasia, nor do they respond to growth hormone, suggesting they exhibit determinate growth like mammals. These results demonstrate that giant danio and zebrafish can be utilized as a direct comparative model system for muscle growth studies, with zebrafish serving as a model organism for determinate growth and giant danio for indeterminate growth.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping