PUBLICATION
A BAC library for the goldfish Carassius auratus auratus (Cyprinidae, Cypriniformes)
- Authors
- Luo, J., Lang, M., Salzburger, W., Siegel, N., Stolting, K.N., and Meyer, A.
- ID
- ZDB-PUB-060531-5
- Date
- 2006
- Source
- Journal of experimental zoology. Part B, Molecular and developmental evolution 306(6): 567-574 (Journal)
- Registered Authors
- Meyer, Axel
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Chromosomes, Artificial, Bacterial*
- DNA Primers
- Evolution, Molecular*
- Gene Library
- Genes, Duplicate/genetics
- Genomics
- Goldfish/genetics*
- Homeodomain Proteins/genetics
- Likelihood Functions
- Models, Genetic
- Molecular Sequence Data
- Phylogeny*
- Sequence Analysis, DNA
- PubMed
- 16708366 Full text @ J. Exp. Zool. B Mol. Dev. Evol.
Citation
Luo, J., Lang, M., Salzburger, W., Siegel, N., Stolting, K.N., and Meyer, A. (2006) A BAC library for the goldfish Carassius auratus auratus (Cyprinidae, Cypriniformes). Journal of experimental zoology. Part B, Molecular and developmental evolution. 306(6):567-574.
Abstract
A goldfish (Carassius auratus auratus) bacterial artificial chromosome genomic library (BAC library) was constructed from one aquarium-bred male specimen (tetraploid, 4n=100, genome size=3.52 pg/cell). The library consists of 128,352 positive clones with an average insert size of 150.4 kb, covering the genome 11-fold. All clones were spotted onto nylon filters and thus are available for screening of genomic regions of interest, such as candidate genes, gene families, or large-sized syntenic DNA regions of cyprinid species. Preliminary screens with two genes were conducted with hybridizing probes to the genes RAG1 and lgi1. RAG1 is a single-copy gene in zebrafish and is duplicated in C. a. auratus. We found a very close correlation between the number of positive BAC clones and the expected library coverage. Two copies of lgi1 were found in zebrafish. We have detected four different copies in C. a. auratus, not in the expected abundance, which indicates some variation in the coverage of the BAC library. The preliminary screens indicate that many duplicated genes that resulted from the ancient fish-specific genome duplication persist in the tetraploid goldfish genome. Hence, the BAC library will provide a useful resource for the future work on comparative genomics, polyploidy, diploidization, and evolutionary genomics in fishes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping