PUBLICATION
Aromatase in zebrafish: A potential target for endocrine disrupting chemicals
- Authors
- Kallivretaki, E., Eggen, R., Neuhauss, S., Alberti, M., Kausch, U., and Segner, H.
- ID
- ZDB-PUB-060517-30
- Date
- 2006
- Source
- Marine Environmental Research 62 Suppl 1: S187-90 (Journal)
- Registered Authors
- Neuhauss, Stephan
- Keywords
- Aromatase, Zebrafish, Endocrine disruption, Immunohistochemistry, Real time RT-PCR
- MeSH Terms
-
- Animals
- Antibodies/metabolism
- Aromatase/biosynthesis
- Aromatase/drug effects*
- Aromatase/genetics
- Aromatase/immunology
- Blotting, Western/methods
- Brain/enzymology
- Brain Chemistry/drug effects
- Gene Expression Regulation, Enzymologic/drug effects*
- Gonads/enzymology
- Immunohistochemistry
- Male
- RNA, Messenger/drug effects
- RNA, Messenger/metabolism
- Reverse Transcriptase Polymerase Chain Reaction/methods
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/biosynthesis
- Zebrafish Proteins/drug effects*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/immunology
- PubMed
- 16698076 Full text @ Mar. Environ. Res.
Citation
Kallivretaki, E., Eggen, R., Neuhauss, S., Alberti, M., Kausch, U., and Segner, H. (2006) Aromatase in zebrafish: A potential target for endocrine disrupting chemicals. Marine Environmental Research. 62 Suppl 1:S187-90.
Abstract
In zebrafish, two isoforms of the aromatase gene exist, namely cyp19a1 and cyp19a2, expressed predominantly in the gonads and brain, respectively. In this study, we focus on characterizing the specificity of antibodies against the aromatase isoforms, and on (xeno)estrogen-induced changes of individual cyp19a2 mRNA concentrations in the brains of adult male zebrafish. Among three polyclonal antibodies studied, the one against CYP19A2 was found to be specific in Western blots and immunohistochemistry. Real-time RT-PCR analyses revealed strong interindividual variation of cyp19a2 levels in the brains of adult male zebrafish. After a three-week-exposure to (xeno)estrogens, mean values of cyp19a2 mRNA levels tended to increase, with significant induction at 200ng 17beta-estradiol/L, but interindividual variation of cyp19a2 expression was maintained.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping