PUBLICATION
Fertilization triggers localized activation of Src-family protein kinases in the zebrafish egg
- Authors
- Sharma, D., and Kinsey, W.H.
- ID
- ZDB-PUB-060517-29
- Date
- 2006
- Source
- Developmental Biology 295(2): 604-614 (Journal)
- Registered Authors
- Kinsey, William H.
- Keywords
- Fertilization, Zebrafish, Src, Fyn, Protein kinase
- MeSH Terms
-
- Animals
- Cytokinesis
- Enzyme Activation
- Fertilization/physiology*
- Fluorescent Antibody Technique
- Ovum/enzymology
- Ovum/physiology*
- Phosphorylation
- Protein Transport
- Proto-Oncogene Proteins c-fyn/metabolism*
- Tissue Distribution
- Zebrafish
- Zebrafish Proteins/metabolism*
- src-Family Kinases/metabolism*
- PubMed
- 16698010 Full text @ Dev. Biol.
Citation
Sharma, D., and Kinsey, W.H. (2006) Fertilization triggers localized activation of Src-family protein kinases in the zebrafish egg. Developmental Biology. 295(2):604-614.
Abstract
Fertilization triggers activation of Src-family kinases in eggs of various species including marine invertebrates and lower vertebrates. While immunofluorescence studies have localized Src-family kinases to the plasma membrane or cortical cytoplasm, no information is available regarding the extent to which these kinases are activated in different regions of the zygote. The objective of the present study was to detect the subcellular distribution of activated Src-family kinases in the fertilized zebrafish egg. An antibody specific for the active, non-phosphorylated form of Src-family PTKs was used to detect these activated kinases by immunofluorescence. The results demonstrate that Fyn, and possibly other Src family members are activated by dephosphorylation of the C-terminal tyrosine at fertilization. The activated Src-family kinases are asymmetrically distributed around the egg cortex with an area of higher kinase activity localized adjacent to the micropyle near the presumptive animal pole. Fertilization initially caused elevation of kinase activity in the cytoplasm underlying the micropyle, but this quickly spread to involve the entire zygote cortex. Later, during egg activation, formation of the blastodisc involved concentration of active Src-family kinase in the blastodisc cortex. As cytokinesis began, activated Src-family kinases were no longer limited to the cortex, but became more evenly distributed in the clear apical cytoplasm of the blastomeres. The results demonstrate that the cortex of the zebrafish egg is functionally differentiated and that fertilization triggers localized activation of Src-family kinases at the point of sperm entry, which subsequently progresses through the entire egg cortex.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping