PUBLICATION
PCNS: A novel protocadherin required for cranial neural crest migration and somite morphogenesis in Xenopus
- Authors
- Rangarajan, J., Luo, T., and Sargent, T.D.
- ID
- ZDB-PUB-060508-16
- Date
- 2006
- Source
- Developmental Biology 295(1): 206-218 (Journal)
- Registered Authors
- Sargent, Tom
- Keywords
- Somitogenesis, Cadherin, Cell adhesion, Epitheliomesenchymal transition
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cadherins/genetics
- Cadherins/metabolism*
- Cell Adhesion
- Cell Movement
- Central Nervous System/embryology
- Central Nervous System/metabolism
- Embryo, Nonmammalian
- Extremities/embryology*
- Female
- Gene Expression Regulation, Developmental
- Molecular Sequence Data
- Morphogenesis
- Neural Crest/cytology*
- Neural Crest/metabolism
- Oligonucleotides, Antisense
- Sequence Homology, Amino Acid
- Xenopus Proteins/genetics
- Xenopus Proteins/metabolism*
- Xenopus laevis/embryology*
- Xenopus laevis/genetics
- PubMed
- 16674935 Full text @ Dev. Biol.
Citation
Rangarajan, J., Luo, T., and Sargent, T.D. (2006) PCNS: A novel protocadherin required for cranial neural crest migration and somite morphogenesis in Xenopus. Developmental Biology. 295(1):206-218.
Abstract
Protocadherins (Pcdhs), a major subfamily of cadherins, play an important role in specific intercellular interactions in development. These molecules are characterized by their unique extracellular domain (EC) with more than 5 cadherin-like repeats, a transmembrane domain (TM) and a variable cytoplasmic domain. PCNS (Protocadherin in Neural crest and Somites), a novel Pcdh in Xenopus, is initially expressed in the mesoderm during gastrulation, followed by expression in the cranial neural crest (CNC) and somites. PCNS has 65% amino acid identity to Xenopus paraxial protocadherin (PAPC) and 42-49% amino acid identity to Pcdh 8 in human, mouse, and zebrafish genomes. Overexpression of PCNS resulted in gastrulation failure but conferred little if any specific adhesion on ectodermal cells. Loss of function accomplished independently with two non-overlapping antisense morpholino oligonucleotides resulted in failure of CNC migration, leading to severe defects in the craniofacial skeleton. Somites and axial muscles also failed to undergo normal morphogenesis in these embryos. Thus, PCNS has essential functions in these two important developmental processes in Xenopus.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping