Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging
- Yaksi, E., and Friedrich, R.W.
- Nature Methods 3(5): 377-383 (Journal)
- Registered Authors
- Friedrich, Rainer, Yaksi, Emre
- MeSH Terms
- Action Potentials/physiology*
- Calcium Signaling/physiology*
- Cations, Divalent
- Fluorescent Dyes/analysis
- Olfactory Bulb/cytology
- Olfactory Bulb/physiology
- Spectrometry, Fluorescence
- Staining and Labeling
- Time Factors
- 16628208 Full text @ Nat. Methods
Yaksi, E., and Friedrich, R.W. (2006) Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging. Nature Methods. 3(5):377-383.
Methods to record action potential (AP) firing in many individual neurons are essential to unravel the function of complex neuronal circuits in the brain. A promising approach is bolus loading of Ca(2+) indicators combined with multiphoton microscopy. Currently, however, this technique lacks cell-type specificity, has low temporal resolution and cannot resolve complex temporal firing patterns. Here we present simple solutions to these problems. We identified neuron types by colocalizing Ca(2+) signals of a red-fluorescing indicator with genetically encoded markers. We reconstructed firing rate changes from Ca(2+) signals by temporal deconvolution. This technique is efficient, dramatically enhances temporal resolution, facilitates data interpretation and permits analysis of odor-response patterns across thousands of neurons in the zebrafish olfactory bulb. Hence, temporally deconvolved Ca(2+) imaging (TDCa imaging) resolves limitations of current optical recording techniques and is likely to be widely applicable because of its simplicity, robustness and generic principle.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes