PUBLICATION
Alternatively spliced Robo2 isoforms in zebrafish and rat
- Authors
- Dalkic, E., Kuscu, C., Sucularli, C., Aydin, I.T., Akcali, K.C., and Konu, O.
- ID
- ZDB-PUB-060424-4
- Date
- 2006
- Source
- Development genes and evolution 216(9): 555-563 (Journal)
- Registered Authors
- Keywords
- Danio rerio, Rattus norvegicus, robo2, Alternative splicing, Differential expression
- MeSH Terms
-
- Alternative Splicing*
- Amino Acid Sequence
- Animals
- Conserved Sequence
- Exons/genetics
- Female
- Gene Expression
- Male
- Molecular Sequence Data
- Protein Isoforms/chemistry
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- RNA, Messenger/analysis
- RNA, Messenger/metabolism
- Rats
- Rats, Sprague-Dawley
- Receptors, Immunologic/chemistry
- Receptors, Immunologic/genetics
- Receptors, Immunologic/metabolism*
- Sequence Alignment
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 16625395 Full text @ Dev. Genes Evol.
Citation
Dalkic, E., Kuscu, C., Sucularli, C., Aydin, I.T., Akcali, K.C., and Konu, O. (2006) Alternatively spliced Robo2 isoforms in zebrafish and rat. Development genes and evolution. 216(9):555-563.
Abstract
Robo2, a member of the robo gene family, functions as a repulsive axon guidance receptor as well as a regulator of cell migration and tissue morphogenesis in different taxa. In this study, a novel isoform of the zebrafish robo2 (robo2_tv2), which included an otherwise alternatively spliced exon (CAE), has been characterized. Robo2_tv2 is expressed differentially in most non-neuronal tissues of adult zebrafish whereas robo2_tv1 expression to a great extent is restricted to the brain and eye. In zebrafish, robo2_tv2 exhibits a very-low-level basal expression starting from 1 day post fertilization until the mid-larval stages, at which time its expression increases dramatically and could be detected throughout adulthood. Our findings demonstrate that the amino acid sequence coded by CAE of the robo2 gene is highly conserved between zebrafish and mammals, and also contains conserved motifs shared with robo1 and robo4 but not with robo3. Furthermore, we provide an account of differential transcription of the CAE homolog in various tissues of the adult rat. These results suggest that the alternatively spliced robo2 isoforms may exhibit tissue specificity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping