Fgf21 is essential for haematopoiesis in zebrafish
- Yamauchi, H., Hotta, Y., Konishi, M., Miyake, A., Kawahara, A., and Itoh, N.
- EMBO reports 7(6): 649-654 (Journal)
- Registered Authors
- Itoh, Nobuyuki, Kawahara, Atsuo, Konishi, Morichika, Miyake, Ayumi
- MeSH Terms
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/physiology
- Erythroid Precursor Cells/physiology
- Fibroblast Growth Factors/genetics
- Fibroblast Growth Factors/physiology*
- Hematopoietic Stem Cells/physiology
- Models, Biological
- Oligonucleotides, Antisense/pharmacology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- 16612391 Full text @ EMBO Rep.
Yamauchi, H., Hotta, Y., Konishi, M., Miyake, A., Kawahara, A., and Itoh, N. (2006) Fgf21 is essential for haematopoiesis in zebrafish. EMBO reports. 7(6):649-654.
Fibroblast growth factors (Fgfs) function as key secreted signalling molecules in many developmental events. The zebrafish is a powerful model system for the investigation of embryonic vertebrate haematopoiesis. Although the effects of Fgf signalling on haematopoiesis in vitro have been reported, the functions of Fgf signalling in haematopoiesis in vivo remain to be explained. We identified Fgf21 in zebrafish embryos. Fgf21-knockdown zebrafish embryos lacked erythroid and myeloid cells but not blood vessels and lymphoid cells. The knockdown embryos had haemangioblasts and haematopoietic stem cells. However, the knockdown embryos had significantly fewer myeloid and erythroid progenitor cells. In contrast, Fgf21 had no significant effect on cell proliferation and apoptosis in the intermediate cell mass. These results indicate that Fgf21 is a newly identified factor essential for the determination of myelo-erythroid progenitor cell fate in vivo.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes