PUBLICATION
Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section
- Authors
- Tsai, T.H., Lin, C.Y., Tsai, H.J., Chen, S.Y., Tai, S.P., Lin, K.H., and Sun, C.K.
- ID
- ZDB-PUB-060412-14
- Date
- 2006
- Source
- Optics letters 31(7): 930-932 (Journal)
- Registered Authors
- Tsai, Huai-Jen
- Keywords
- none
- MeSH Terms
-
- Zebrafish
- Tissue Distribution
- Image Enhancement/methods*
- Heart/embryology*
- Animals
- Microscopy, Fluorescence, Multiphoton/methods*
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism*
- Myocardium/cytology*
- Myocardium/metabolism*
- Recombinant Fusion Proteins/metabolism*
- Biopolymers/metabolism
- PubMed
- 16599215 Full text @ Opt. Lett.
Citation
Tsai, T.H., Lin, C.Y., Tsai, H.J., Chen, S.Y., Tai, S.P., Lin, K.H., and Sun, C.K. (2006) Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section. Optics letters. 31(7):930-932.
Abstract
Received October 14, 2005; revised January 7, 2006; accepted January 9, 2006; posted January 12, 2006 (Doc. ID 65391) The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wavelength in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping