ZFIN ID: ZDB-PUB-060412-14
Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section
Tsai, T.H., Lin, C.Y., Tsai, H.J., Chen, S.Y., Tai, S.P., Lin, K.H., and Sun, C.K.
Date: 2006
Source: Optics letters   31(7): 930-932 (Journal)
Registered Authors: Tsai, Huai-Jen
Keywords: none
MeSH Terms:
  • Animals
  • Biopolymers/metabolism
  • Heart/embryology*
  • Image Enhancement/methods*
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism*
  • Microscopy, Fluorescence, Multiphoton/methods*
  • Myocardium/cytology*
  • Myocardium/metabolism*
  • Recombinant Fusion Proteins/metabolism*
  • Tissue Distribution
  • Zebrafish
PubMed: 16599215 Full text @ Opt. Lett.
Received October 14, 2005; revised January 7, 2006; accepted January 9, 2006; posted January 12, 2006 (Doc. ID 65391) The two-photon excitation action cross section of Hc-Red fluorescent proteins (Hc-RFPs) is measured and found to be of the same order as that of enhanced green fluorescent proteins. With a 618 nm emission wavelength in the far-red region and with an excitation wavelength around 1200 nm, Hc-RPF-based two-photon fluorescence microscopy (2PFM) can offer deep penetration capability inside live samples and is ideal for in vivo gene expression study and biomolecular imaging in live objects. In vivo 2PFM of the developing heart deep inside a transgenic zebrafish embryo tagged by Hc-RFP is also successfully demonstrated.