PUBLICATION
Frequency-dependent modulation of glycine receptor activation recorded from the zebrafish larvae hindbrain
- Authors
- Rigo, J.M., and Legendre, P.
- ID
- ZDB-PUB-060403-2
- Date
- 2006
- Source
- Neuroscience 140(2): 389-402 (Journal)
- Registered Authors
- Legendre, Pascal
- Keywords
- glycine receptors, patch-clamp, desensitization, frequency, synaptic activity, modulation
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Down-Regulation/drug effects
- Down-Regulation/physiology
- Efferent Pathways/cytology
- Efferent Pathways/drug effects
- Efferent Pathways/metabolism
- Electric Stimulation
- Glycine/metabolism*
- Glycine/pharmacology
- Markov Chains
- Membrane Potentials/drug effects
- Membrane Potentials/physiology
- Models, Neurological
- Neural Inhibition/drug effects
- Neural Inhibition/physiology*
- Neurons/drug effects
- Neurons/metabolism*
- Patch-Clamp Techniques
- Receptors, Glycine/drug effects
- Receptors, Glycine/metabolism*
- Reticular Formation/cytology
- Reticular Formation/drug effects
- Reticular Formation/metabolism
- Rhombencephalon/cytology
- Rhombencephalon/drug effects
- Rhombencephalon/metabolism*
- Synaptic Transmission/drug effects
- Synaptic Transmission/physiology*
- PubMed
- 16564635 Full text @ Neuroscience
Citation
Rigo, J.M., and Legendre, P. (2006) Frequency-dependent modulation of glycine receptor activation recorded from the zebrafish larvae hindbrain. Neuroscience. 140(2):389-402.
Abstract
In vertebrates, most glycinergic inhibitory neurons discharge phasically at a relatively low frequency. Such a pattern of glycine liberation from presynaptic terminals may affect the kinetics of post-synaptic glycine receptors. To examine this influence, we have analyzed the behavior of glycine receptors in response to repetitive stimulation at frequencies at which consecutive outside-out currents did not superimpose (0.5-4 Hz). Neurotransmitter release was mimicked on outside-out patches from zebrafish hindbrain Mauthner cells using fast flow application techniques. The amplitude of outside-out currents evoked by short (1 ms) repetitive applications of a saturating concentration (3 mM) of glycine remained unchanged for application frequencies 1 Hz. Glycine-evoked current simulations using a simple Markov model describing zebrafish glycine receptor kinetic behavior, indicates that this down-regulation of glycine receptor efficacy is due to a progressive accumulation of the receptors in a long lasting desensitization state. Our simulations suggest that this down-regulation can occur even when spontaneous inhibitory currents were generated randomly at a frequency >1 Hz.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping