PUBLICATION
HuC:Kaede, a useful tool to label neural morphologies in networks in vivo
- Authors
- Sato, T., Takahoko, M., and Okamoto, H.
- ID
- ZDB-PUB-060306-6
- Date
- 2006
- Source
- Genesis (New York, N.Y. : 2000) 44(3): 136-142 (Journal)
- Registered Authors
- Okamoto, Hitoshi, Sato, Tomomi
- Keywords
- neuronal labeling, photo-conversion, transgenic, zebrafish
- MeSH Terms
-
- Zebrafish Proteins/genetics*
- Zebrafish/embryology
- ELAV Proteins/genetics*
- Luminescent Proteins/genetics*
- Gene Expression Regulation, Developmental/physiology*
- Nerve Net/cytology
- Nerve Net/embryology*
- Recombinant Fusion Proteins/metabolism
- Neurons/cytology*
- Transgenes
- Embryo, Nonmammalian/metabolism
- Animals
- Trigeminal Ganglion/cytology
- Trigeminal Ganglion/embryology*
- Staining and Labeling/methods
- Microinjections
- ELAV-Like Protein 3
- Dendrites/physiology
- PubMed
- 16496337 Full text @ Genesis
Citation
Sato, T., Takahoko, M., and Okamoto, H. (2006) HuC:Kaede, a useful tool to label neural morphologies in networks in vivo. Genesis (New York, N.Y. : 2000). 44(3):136-142.
Abstract
A simple and efficient procedure for labeling neurons is a prerequisite for investigating the development of neural networks in zebrafish. To label neurons we used Kaede, a fluorescent protein with a photoconversion property allowing conversion from green to red fluorescence following irradiation with UV or violet light. We established a zebrafish stable transgenic line, Tg(HuC:Kaede), expressing Kaede in neurons under the control of the HuC promoter. This transgenic line was used to label a small number of neurons in the trigeminal ganglion. Also, using embryos injected with the transgene, we labeled peripheral axon arbors of a Rohon-Beard neuron at 4 days postfertilization and observed the dendrite development of a tectal neuron for 3 days. These data indicate that Kaede is a useful tool to selectively label neural networks in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping