ZFIN ID: ZDB-PUB-060105-3
In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes
Kloosterman, W.P., Wienholds, E., de Bruijn, E., Kauppinen, S., and Plasterk, R.H.
Date: 2006
Source: Nature Methods   3(1): 27-29 (Journal)
Registered Authors: de Bruijn, Ewart, Kloosterman, Wigard, Plasterk, Ronald H.A., Wienholds, Erno
Keywords: none
MeSH Terms:
  • Animals
  • Embryo, Mammalian/chemistry*
  • Embryo, Nonmammalian/chemistry
  • Gene Expression Profiling/methods
  • In Situ Hybridization/methods*
  • Mice
  • MicroRNAs/analysis*
  • Oligonucleotide Probes/chemistry*
  • Oligonucleotides
  • Oligonucleotides, Antisense/chemistry*
  • Zebrafish/genetics
PubMed: 16369549 Full text @ Nat. Methods
MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.