PUBLICATION

In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes

Authors
Kloosterman, W.P., Wienholds, E., de Bruijn, E., Kauppinen, S., and Plasterk, R.H.
ID
ZDB-PUB-060105-3
Date
2006
Source
Nature Methods   3(1): 27-29 (Journal)
Registered Authors
de Bruijn, Ewart, Kloosterman, Wigard, Plasterk, Ronald H.A., Wienholds, Erno
Keywords
none
MeSH Terms
  • Animals
  • Embryo, Mammalian/chemistry*
  • Embryo, Nonmammalian/chemistry
  • Gene Expression Profiling/methods
  • In Situ Hybridization/methods*
  • Mice
  • MicroRNAs/analysis*
  • Oligonucleotide Probes/chemistry*
  • Oligonucleotides
  • Oligonucleotides, Antisense/chemistry*
  • Zebrafish/genetics
PubMed
16369549 Full text @ Nat. Methods
Abstract
MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping