PUBLICATION

Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones

Authors

Kang, J.S., Oohashi, T., Kawakami, Y., Bekku, Y., Izpisua, B.J.C., and Ninomiya, Y.

ID

ZDB-PUB-051229-1

Date

2004

Source

Mechanisms of Development 121(3): 301-312 (Journal)

Registered Authors

Kawakami, Yasuhiko, Oohashi, Toshi

Keywords

Dermacan; Aggrecan; Versican; Lectican; Opercle; Branchiostegal ray; Dermal bone; Craniofacial development; Morpholino; Zebrafish

MeSH Terms

Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/growth & development
Proteoglycans/genetics*
Proteoglycans/metabolism
Proteoglycans/physiology*
Sequence Alignment
Amino Acid Sequence
Base Sequence
Facial Bones/embryology
Facial Bones/growth & development
Facial Bones/metabolism
Phylogeny
Larva/drug effects
Molecular Sequence Data
Gene Expression
In Situ Hybridization, Fluorescence
Bone Development*/genetics
Chondroitin Sulfate Proteoglycans/metabolism
Zebrafish Proteins/genetics*
Zebrafish Proteins/metabolism
Zebrafish Proteins/physiology*
Extracellular Matrix Proteins/metabolism
Animals
Aggrecans
Versicans
Lectins, C-Type

(all 27)

PubMed

15003632 Full text @ Mech. Dev.

Abstract

We report here the isolation and characterization of a cDNA encoding zebrafish dermacan, a novel member of hyaluronan (HA)-binding proteoglycans, which was termed after its characteristic expression in the zebrafish dermal bones. The deduced protein sequence shares the typical modular elements of lecticans. Sequence comparison covering the C-terminal globular domain demonstrated that dermacan shows high homology with zebrafish versican but is distinct from any other identified lecticans. Genomic DNA analysis demonstrated that dermacan and versican were encoded by distinct genes in the zebrafish genome. The expression of dermacan is initiated in the sclerotome and cephalic paraxial mesoderm at 16 h postfertilization. During the pharyngular period, dermacan transcripts were detected in the sclerotome, tail fin bud, pharyngular arch primordial region, and otic vesicle. In the development of craniofacial bones, dermacan expression was detected typically in the opercle and dentary. These regions belong to the craniofacial dermal bones. aggrecan expression, in contrast, was observed in the elements of craniofacial cartilage bones. In the dermacan–morpholino–injected embryos, dermal bones, e.g. opercle, dentary, and branchiostegal rays, as well as axial skeleton in the trunk, showed decreased ossification. We conclude that dermacan is a novel lectican gene, and that zebrafish lectican genes have genetically diverged. In addition, our data suggest the involvement of dermacan in zebrafish dermal bone development.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
acana	WT	standard conditions	Day 5	basibranchial	ISH	Fig. 6
acana	WT	standard conditions	Day 5	ceratobranchial bone	ISH	Fig. 6
acana	WT	standard conditions	Day 5	ceratohyal bone	ISH	Fig. 6
acana	WT	standard conditions	Protruding-mouth	chondrocranium	ISH	Fig. 6
acana	WT	standard conditions	Day 5	Meckel's cartilage	ISH	Fig. 6
acana	WT	standard conditions	Day 4	neurocranial trabecula	ISH	Fig. 6
acana	WT	standard conditions	Day 4	olfactory region	ISH	Fig. 6
acana	WT	standard conditions	Protruding-mouth	olfactory region	ISH	Fig. 6
acana	WT	standard conditions	Day 5	pectoral fin	ISH	Fig. 6
acana	WT	standard conditions	Day 4	pectoral fin	ISH	Fig. 6

1 - 10 of 35

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Phenotype

Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
vcanb	MO1-vcanb	MRPHLNO

Fish

Fish
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Kang et al., 2004 ZDB-PUB-051229-1 PMID:15003632

Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones

Kang et al., 2004

ZDB-PUB-051229-1
PMID:15003632