PUBLICATION
The zebrafish dorsal axis is apparent at the four-cell stage
- Authors
- Gore, A.V., Maegawa, S., Cheong, A., Gilligan, P.C., Weinberg, E.S., and Sampath, K.
- ID
- ZDB-PUB-051219-4
- Date
- 2005
- Source
- Nature 438(7070): 1030-1035 (Journal)
- Registered Authors
- Cheong, Albert, Gilligan, Patrick, Gore, Aniket, Maegawa, Shingo, Sampath, Karuna
- Keywords
- none
- MeSH Terms
-
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/embryology*
- Embryo, Nonmammalian/metabolism
- Zebrafish Proteins/genetics*
- beta Catenin/metabolism
- Nodal Signaling Ligands
- Oocytes/metabolism
- 3' Untranslated Regions/genetics
- Base Sequence
- Molecular Sequence Data
- Body Patterning*
- Animals
- Zebrafish/embryology*
- Zebrafish/genetics
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- PubMed
- 16355228 Full text @ Nature
Citation
Gore, A.V., Maegawa, S., Cheong, A., Gilligan, P.C., Weinberg, E.S., and Sampath, K. (2005) The zebrafish dorsal axis is apparent at the four-cell stage. Nature. 438(7070):1030-1035.
Abstract
A central question in the development of multicellular organisms pertains to the timing and mechanisms of specification of the embryonic axes. In many organisms, specification of the dorsoventral axis requires signalling by proteins of the Transforming growth factor-beta and Wnt families. Here we show that maternal transcripts of the zebrafish Nodal-related morphogen, Squint (Sqt), can localize to two blastomeres at the four-cell stage and predict the dorsal axis. Removal of cells containing sqt transcripts from four-to-eight-cell embryos or injection of antisense morpholino oligonucleotides targeting sqt into oocytes can cause a loss of dorsal structures. Localization of sqt transcripts is independent of maternal Wnt pathway function and requires a highly conserved sequence in the 3' untranslated region. Thus, the dorsoventral axis is apparent by early cleavage stages and may require the maternally encoded morphogen Sqt and its associated factors. Because the 3' untranslated region of the human nodal gene can also localize exogenous sequences to dorsal cells, this mechanism may be evolutionarily conserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping