PUBLICATION
A critical role for calponin 2 in vascular development
- Authors
- Tang, J., Hu, G., Hanai, J.I., Yadlapalli, G., Lin, Y., Zhang, B., Galloway, J., Bahary, N., Sinha, S., Thisse, B., Thisse, C., Jin, J.P., Zon, L.I., and Sukhatme, V.P.
- ID
- ZDB-PUB-051207-12
- Date
- 2006
- Source
- The Journal of biological chemistry 281(10): 6664-6672 (Journal)
- Registered Authors
- Bahary, Nathan, Galloway, Jenna, Sinha, Soniya, Sukhatme, Vikas, Thisse, Bernard, Thisse, Christine, Zon, Leonard I.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Calcium-Binding Proteins/physiology*
- Cell Line
- Cell Movement/physiology
- Cloning, Molecular
- Endothelium, Vascular/cytology
- Endothelium, Vascular/physiology*
- Extracellular Signal-Regulated MAP Kinases/metabolism
- Humans
- MAP Kinase Kinase Kinases/metabolism
- Microfilament Proteins/physiology*
- Molecular Sequence Data
- Neovascularization, Physiologic/genetics
- Neovascularization, Physiologic/physiology*
- Oligonucleotides, Antisense
- Phosphorylation
- Signal Transduction/physiology
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/physiology*
- PubMed
- 16317011 Full text @ J. Biol. Chem.
Citation
Tang, J., Hu, G., Hanai, J.I., Yadlapalli, G., Lin, Y., Zhang, B., Galloway, J., Bahary, N., Sinha, S., Thisse, B., Thisse, C., Jin, J.P., Zon, L.I., and Sukhatme, V.P. (2006) A critical role for calponin 2 in vascular development. The Journal of biological chemistry. 281(10):6664-6672.
Abstract
Calponin 2 (h2 calponin, CNN2) is an actin-binding protein implicated in cytoskeletal organization. We have found that the expression of calponin 2 is relatively restricted to vasculature from 16 to 30 hours post fertilization during zebrafish (Danio rerio) development. Forty eight hours after injecting antisense morpholino oligos against calponin 2 into embryos at the 1 to 4-cell stage, zebrafish demonstrated various cardiovascular defects including sluggish axial and head circulation, absence of circulation in intersegmental vessels and in the dorsal longitudinal anastomotic vessel, enlarged cerebral ventricles and pericardial edema, in addition to an excess bending, spiraling tail and twisting of the caudal fin. Knockdown of calponin 2 in the [Tg(fli1:EGFP)y1] zebrafish line (in which a fli1 promoter drives vascular specific EGFP expression) indicated that diminished calponin 2 expression blocked the proper migration of endothelial cells during formation of intersegmental vessels. In vitro studies showed that basic fibroblast growth factor-induced human umbilical vein endothelial cell migration was downregulated by knockdown of calponin 2 expression using an antisense adenovirus, and overexpression of calponin 2 enhanced migration and hastened wound healing. These events were correlated with activation of mitogen-activated protein kinase; moreover, inhibitor of this pathway blocked calponin 2's promigratory effect. Collectively, these data suggest that calponin 2 plays an important role in migration of endothelial cells both in vivo and in vitro and that its expression is critical for proper vascular development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping