PUBLICATION

Long-Term Time-Lapse Fluorescence Imaging of Developing Zebrafish

Authors
Kamei, M., and Weinstein, B.M.
ID
ZDB-PUB-051121-3
Date
2005
Source
Zebrafish   2(2): 113-123 (Journal)
Registered Authors
Kamei, Makoto, Weinstein, Brant M.
Keywords
none
MeSH Terms
none
PubMed
18248171 Full text @ Zebrafish
Abstract
A variety of different transgenic zebrafish lines have been generated expressing green fluorescent protein (GFP) or other fluorescent proteins in different organs and tissues, permitting dynamic visualization of development of these organs and tissues in living animals via time-lapse imaging. Although methods have been devised for shortto medium-term time-lapse imaging of transgenic zebrafish, these methods are not suitable for longer term imaging because of poor control over temperature, evaporation, and anoxia. We describe a new imaging chamber that provides continuously circulating flow of warm, oxygenated aqueous media. We show that the chamber can be used for multiphoton time-lapse imaging of developing blood vessels in the trunk of Fli1-EGFP transgenic zebrafish for 5 days without developmental delay, loss of viability, or evident reduction in strength of circulatory flow. This imaging chamber provides an important new tool for long-term dynamic imaging of transgenic zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes