PUBLICATION

Cellular and molecular analyses of vascular tube and lumen formation in zebrafish

Authors
Jin, S.W., Beis, D., Mitchell, T., Chen, J.N., Stainier, D.Y.
ID
ZDB-PUB-051031-2
Date
2005
Source
Development (Cambridge, England)   132(23): 5199-5209 (Journal)
Registered Authors
Beis, Dimitris, Chen, Jau-Nian, Jin, Suk-Won, Stainier, Didier
Keywords
Endothelial cell, Migration, Endoderm, VEGF, Angioblast, Zebrafish
MeSH Terms
  • Cell Movement
  • Green Fluorescent Proteins/genetics
  • Vascular Endothelial Growth Factor Receptor-2/genetics
  • Endothelial Cells/cytology
  • Embryo, Nonmammalian
  • Intercellular Junctions/physiology
  • Animals, Genetically Modified
  • Endoderm/physiology
  • Zebrafish
  • Stem Cells/cytology
  • Blood Vessels/cytology*
  • Blood Vessels/embryology
  • Blood Vessels/growth & development*
  • Body Patterning
  • Promoter Regions, Genetic
  • Animals
(all 16)
PubMed
16251212 Full text @ Development
Abstract
Tube and lumen formation are essential steps in forming a functional vasculature. Despite their significance, our understanding of these processes remains limited, especially at the cellular and molecular levels. In this study, we analyze mechanisms of angioblast coalescence in the zebrafish embryonic midline and subsequent vascular tube formation. To facilitate these studies, we generated a transgenic line where EGFP expression is controlled by the zebrafish flk1 promoter. We find that angioblasts migrate as individual cells to form a vascular cord at the midline. This transient structure is stabilized by endothelial cell-cell junctions, and subsequently undergoes lumen formation to form a fully patent vessel. Downregulating the VEGF signaling pathway, while affecting the number of angioblasts, does not appear to affect their migratory behavior. Our studies also indicate that the endoderm, a tissue previously implicated in vascular development, provides a substratum for endothelial cell migration and is involved in regulating the timing of this process, but that it is not essential for the direction of migration. In addition, the endothelial cells in endodermless embryos form properly lumenized vessels, contrary to what has been previously reported in Xenopus and avian embryos. These studies provide the tools and a cellular framework for the investigation of mutations affecting vasculogenesis in zebrafish.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m425
    		Point Mutation
    ne2067TgTransgenic Insertion
      s4
        		Point Mutation
        s843TgTransgenic Insertion
          s844TgTransgenic Insertion
            s849TgTransgenic Insertion
              y1TgTransgenic Insertion
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                plcg1MO3-plcg1MRPHLNO
                vegfaaMO3-vegfaaMRPHLNO
                1 - 2 of 2
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                Fish
                Fish
                mixl1m425/m425 (AB)
                ne2067Tg
                s843Tg
                s849Tg
                sox32s4/s4
                WT
                WT + MO3-plcg1
                WT + MO3-vegfaa
                y1Tg
                1 - 9 of 9
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                Antibodies
                No data available
                Orthology
                No data available
                Engineered Foreign Genes
                No data available
                Mapping
                Entity Type Entity Symbol Location
                Features843TgChr: 16 Details
                1 - 1 of 1
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                Citation
                Jin, S.W., Beis, D., Mitchell, T., Chen, J.N., Stainier, D.Y. (2005) Cellular and molecular analyses of vascular tube and lumen formation in zebrafish. Development (Cambridge, England). 132(23):5199-5209.