PUBLICATION
Study on fish embryo responses to the treatment of cryoprotective chemicals using impedance spectroscopy
- Authors
- Wang, R.Y., Zhang, T., Bao, Q., and Rawson, D.M.
- ID
- ZDB-PUB-051031-13
- Date
- 2006
- Source
- European biophysics journal : EBJ 35(3): 224-230 (Journal)
- Registered Authors
- Rawson, David M., Zhang, Tiantian
- Keywords
- Impedance spectroscopy, Cryopreservation, Embryos, Membrane permeability, Zebrafish, Danio rerio, Normalised permittivity, Conductivity
- MeSH Terms
-
- Animals
- Cell Membrane Permeability*/drug effects
- Cryopreservation/methods
- Cryoprotective Agents*/administration & dosage
- Cryoprotective Agents*/metabolism
- Dimethyl Sulfoxide/administration & dosage
- Dimethyl Sulfoxide/metabolism
- Electric Conductivity*
- Electric Impedance*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Female
- Methanol/administration & dosage
- Methanol/metabolism
- Sensitivity and Specificity
- Spectrum Analysis/methods
- Zebrafish*/embryology
- PubMed
- 16244882 Full text @ Eur. Biophy. J.
Citation
Wang, R.Y., Zhang, T., Bao, Q., and Rawson, D.M. (2006) Study on fish embryo responses to the treatment of cryoprotective chemicals using impedance spectroscopy. European biophysics journal : EBJ. 35(3):224-230.
Abstract
Investigations using electrical impedance spectroscopy to measure the responses of fish embryos to the cryoprotective chemicals, methanol and dimethyl sulphoxide (DMSO), were carried out. Zebrafish (Danio rerio) embryos were used as a model to study the newly proposed technique. The normalised permittivity and conductivity changes of the embryos were measured continuously over a 20-min period in a customised embryo-holding chamber. The normalised permittivity and conductivity spectra were obtained during embryo exposure to different concentrations of methanol (1.0, 2.0 and 3.0 M) and DMSO (0.5, 1.0 and 2.0 M) solutions. The results showed significant permittivity and conductivity changes after embryo exposure to methanol and DMSO at the optimum embryo loading level (six embryos). Embryos in different concentrations of methanol and DMSO also resulted in quantitative responses shown in the normalised permittivity and conductivity spectra. The results demonstrated that fish embryo membrane permeability to cryoprotective chemicals could be monitored in real-time. The measurement of permittivity at a lower frequency range (10-10(3) Hz) and conductivity at a higher frequency range (10(4)-10(6) Hz) during fish embryo exposure to cryoprotective chemicals using impedance spectroscopy can be used as a new tool for the fast screening of most effective cryoprotective chemicals. The results from the present study also demonstrated the possibility of quantifying the level of cryoprotective chemicals penetrating the fish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping