|ZFIN ID: ZDB-PUB-051019-5|
Zebrafish KLF4 is essential for anterior mesendoderm/pre-polster differentiation and hatching
Gardiner, M.R., Daggett, D.F., Zon, L.I., and Perkins, A.C.
|Source:||Developmental dynamics : an official publication of the American Association of Anatomists 234(4): 992-996 (Journal)|
|Registered Authors:||Daggett, Dave, Perkins, Andrew, Zon, Leonard I.|
|Keywords:||BMP4, Cap1, CatL, hatching gland, anterior mesendoderm, polster, zebrafish, zKLF4|
|PubMed:||16222715 Full text @ Dev. Dyn.|
Gardiner, M.R., Daggett, D.F., Zon, L.I., and Perkins, A.C. (2005) Zebrafish KLF4 is essential for anterior mesendoderm/pre-polster differentiation and hatching. Developmental dynamics : an official publication of the American Association of Anatomists. 234(4):992-996.
ABSTRACTGene knockout studies of Kruppel-like factors (KLFs) in mice have shown essential roles in organogenesis. A screen for KLF family members in zebrafish identified many KLFs. One of these, zebrafish KLF4 (zKLF4) is the homologue of neptune, a Xenopus laevis KLF. zKLF4 is expressed from approximately 80% epiboly a patch of dorsal/anterior mesendodermal cells called the pre-polster and, subsequently, in the polster and hatching gland. Here we investigate the function of zKLF4 using morpholino-based antisense oligonucleotides. Knockdown of zKLF4 resulted in complete absence of hatching gland formation and subsequent hatching in zebrafish. In addition, there was early knockdown of expression of the pre-polster/anterior mesendoderm markers CatL, cap1, and BMP4. These results indicate zKLF4 is expressed within the pre-polster, an early mesendodermal site, and that it plays a critical role in the differentiation of these cells into hatching gland cells.