PUBLICATION

Neuropilin-1a is involved in trunk motor axon outgrowth in embryonic zebrafish

Authors
Feldner, J., Becker, T., Goishi, K., Schweitzer, J., Lee, P., Schachner, M., Klagsbrun, M., and Becker, C.G.
ID
ZDB-PUB-050823-8
Date
2005
Source
Developmental dynamics : an official publication of the American Association of Anatomists   234(3): 535-549 (Journal)
Registered Authors
Becker, Catherina G., Becker, Thomas, Feldner, Julia, Goishi, Katsutoshi, Schachner, Melitta, Schweitzer, Jörn
Keywords
axon guidance, angiogenesis, semaphorin, VEGF, primary motor neurons
MeSH Terms
  • Animals
  • Axons/drug effects
  • Axons/physiology*
  • Gene Expression Regulation, Developmental/drug effects
  • Motor Neurons/drug effects
  • Motor Neurons/metabolism*
  • Neuropilin-1/genetics
  • Neuropilin-1/metabolism*
  • Phenotype
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Semaphorin-3A/metabolism
  • Vascular Endothelial Growth Factor A/pharmacology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed
16110501 Full text @ Dev. Dyn.
Abstract
Neuropilin-1, a receptor for axon-repellent semaphorins and vascular endothelial growth factor (VEGF), functions both in angiogenesis and axon growth. Here, we show strong expression of neuropilin-1a in primary motor neurons in the trunk of embryonic zebrafish. Reducing the expression of neuropilin-1a using antisense morpholino oligonucleotides induced aberrant branching of motor nerves, additional exit points of motor nerves from the spinal cord, and migration of neurons out of the spinal cord along the motor axon pathway in a dose-dependent manner. These phenotypes could be partially rescued by coinjecting neuropilin-1a mRNA. Other axons in the spinal cord and head appeared unaffected by the morpholino treatment. In addition, neuropilin-1a morpholino treatment disturbed normal formation of blood vessels in the trunk of 24 hours postfertilization embryos, as shown by microangiography. Morpholinos to VEGF also disturbed formation of blood vessels but did not affect motor axons, indicating that correct formation of blood vessels is not needed for the growth of primary motor axons. Morpholinos to the semaphorin 3A homologs semaphorin 3A1 and semaphorin 3A2 also had no effect on motor axon growth. However, combined injections of neuropilin-1a morpholino, at a concentration that did not elicit axonal aberrations when injected alone, with VEGF, semaphorin 3A1, or semaphorin 3A2 morpholinos synergistically increased the proportion of embryos showing aberrant motor axon growth. Thus, neuropilin-1a in primary motor neurons may integrate signals from several ligands and is needed for proper segmental growth of primary motor nerves in zebrafish.
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