PUBLICATION

Transposon tools and methods in zebrafish

Authors
Kawakami, K.
ID
ZDB-PUB-050823-10
Date
2005
Source
Developmental dynamics : an official publication of the American Association of Anatomists   234(2): 244-254 (Review)
Registered Authors
Kawakami, Koichi
Keywords
zebrafish, transposon, gene trapping, enhancer trapping, insertional mutagenesis
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • DNA Transposable Elements*
  • Enhancer Elements, Genetic
  • Exons
  • Genetic Techniques
  • Genome
  • Genomics
  • Models, Genetic*
  • Mutagenesis*
  • Mutagenesis, Insertional
  • Zebrafish
PubMed
16110506 Full text @ Dev. Dyn.
Abstract
Zebrafish is an excellent model animal to study vertebrate development by genetic approaches. Hundreds of mutations affecting various processes of development have been isolated by chemical mutagenesis and insertional mutagenesis using a pseudotyped retrovirus. However, useful transposon tools and methods had not been available in zebrafish. This is mainly because no active transposable element has been found from the zebrafish genome. Recently, efficient transgenesis, gene trap, and enhancer trap methods have been developed in zebrafish by using the Tol2 and the Sleeping Beauty transposon systems. These methods should increase the usefulness of zebrafish as a model vertebrate and facilitate the study of developmental biology, genetics, and genomics.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes