PUBLICATION
Transposon tools and methods in zebrafish
- Authors
- Kawakami, K.
- ID
- ZDB-PUB-050823-10
- Date
- 2005
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 234(2): 244-254 (Review)
- Registered Authors
- Kawakami, Koichi
- Keywords
- zebrafish, transposon, gene trapping, enhancer trapping, insertional mutagenesis
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- DNA Transposable Elements*
- Enhancer Elements, Genetic
- Exons
- Genetic Techniques
- Genome
- Genomics
- Models, Genetic*
- Mutagenesis*
- Mutagenesis, Insertional
- Zebrafish
- PubMed
- 16110506 Full text @ Dev. Dyn.
Citation
Kawakami, K. (2005) Transposon tools and methods in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 234(2):244-254.
Abstract
Zebrafish is an excellent model animal to study vertebrate development by genetic approaches. Hundreds of mutations affecting various processes of development have been isolated by chemical mutagenesis and insertional mutagenesis using a pseudotyped retrovirus. However, useful transposon tools and methods had not been available in zebrafish. This is mainly because no active transposable element has been found from the zebrafish genome. Recently, efficient transgenesis, gene trap, and enhancer trap methods have been developed in zebrafish by using the Tol2 and the Sleeping Beauty transposon systems. These methods should increase the usefulness of zebrafish as a model vertebrate and facilitate the study of developmental biology, genetics, and genomics.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping