PUBLICATION
Differences in the regenerative response of neuronal cell populations and indications for plasticity in intraspinal neurons after spinal cord transection in adult zebrafish
- Authors
- Becker, T., Lieberoth, B.C., Becker, C.G., and Schachner, M.
- ID
- ZDB-PUB-050818-8
- Date
- 2005
- Source
- Molecular and cellular neurosciences 30(2): 265-278 (Journal)
- Registered Authors
- Becker, Catherina G., Becker, Thomas, Lieberoth, Bettina, Schachner, Melitta
- Keywords
- none
- MeSH Terms
-
- Animals
- Biomarkers
- Cell Division
- GAP-43 Protein/analysis
- GAP-43 Protein/genetics
- Ganglia, Spinal/physiology*
- In Situ Hybridization
- Nerve Regeneration/physiology*
- Neurons/cytology
- Neurons/physiology*
- RNA, Messenger
- Spinal Cord/physiology*
- Zebrafish
- PubMed
- 16098761 Full text @ Mol. Cell Neurosci.
Citation
Becker, T., Lieberoth, B.C., Becker, C.G., and Schachner, M. (2005) Differences in the regenerative response of neuronal cell populations and indications for plasticity in intraspinal neurons after spinal cord transection in adult zebrafish. Molecular and cellular neurosciences. 30(2):265-278.
Abstract
In zebrafish, the capacity to regenerate long axons varies among different populations of axotomized neurons after spinal cord transection. In specific brain nuclei, 84-92% of axotomized neurons upregulate expression of the growth-related genes GAP-43 and L1.1 and 32-51% of these neurons regrow their descending axons. In contrast, 16-31% of spinal neurons with axons ascending to the brainstem upregulate these genes and only 2-4% regrow their axons. Dorsal root ganglion (DRG) neurons were not observed to regrow their ascending axons or to increase expression of GAP-43 mRNA. Expression of L1.1 mRNA is high in unlesioned and axotomized DRG neurons. In the lesioned spinal cord, expression of growth-related molecules is increased in a substantial population of non-axotomized neurons, suggesting morphological plasticity in the spinal-intrinsic circuitry. We propose that locomotor recovery in spinal-transected adult zebrafish is influenced less by recovery of ascending pathways, but more by regrowth of descending tracts and rearrangement of intraspinal circuitry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping