PUBLICATION

Large-scale enhancer detection in the zebrafish genome

Authors
Ellingsen, S., Laplante, M.A., Konig, M., Kikuta, H., Furmanek, T., Hoivik, E.A., and Becker, T.S.
ID
ZDB-PUB-050803-4
Date
2005
Source
Development (Cambridge, England)   132(17): 3799-3811 (Journal)
Registered Authors
Becker, Thomas S., Ellingsen, Ståle, Kikuta, Hiroshi, Laplante, Mary
Keywords
Cis-regulatory sequence, Synteny, In vivo imaging, Zebrafish
MeSH Terms
  • Alleles
  • Animals
  • Animals, Genetically Modified
  • Cells, Cultured
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Enhancer Elements, Genetic/genetics*
  • Gene Expression Regulation, Developmental/genetics
  • Genes, Reporter/genetics
  • Genome*
  • Mutagenesis, Insertional
  • Transcription, Genetic/genetics
  • Zebrafish/genetics*
PubMed
16049110 Full text @ Development
Abstract
Murine retroviral vectors carrying an enhancer detection cassette were used to generate 95 transgenic lines of fish in which reporter expression is observed in distinct patterns during embryonic development. We mapped 65 insertion sites to the as yet unfinished zebrafish genome sequence. Many integrations map close to previously known developmental genes, including transcription factors of the Pax, Hox, Sox, Pou, Otx, Emx, zinc-finger and bHLH gene families. In most cases, the activated provirus is located in, or within a 15 kb interval around, the corresponding transcriptional unit. The exceptions include four insertions into a gene desert on chromosome 20 upstream of sox11b, and an insertion upstream of otx1. In these cases, the activated insertions are found at a distance of between 32 kb and 132 kb from the coding region. These as well as seven other insertions described here identify genes that have recently been associated with ultra conserved non-coding elements found in all vertebrate genomes.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes