PUBLICATION
Characterization of Promoter Activities of Four Different Japanese Flounder Promoters in Transgenic Zebrafish
- Authors
- Yazawa, R., Hirono, I., and Aoki, T.
- ID
- ZDB-PUB-050727-9
- Date
- 2005
- Source
- Marine biotechnology (New York, N.Y.) 7(6): 625-633 (Journal)
- Registered Authors
- Aoki, Takashi, Hirono, Ikuo
- Keywords
- Japanese flounder, tissue-specific promoter, inducible promoter, green fluorescence protein (GFP), transgenic zebrafish
- MeSH Terms
-
- Liver/metabolism
- Zebrafish
- Matrix Metalloproteinase 9/genetics
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Gene Expression Regulation*
- Promoter Regions, Genetic/genetics*
- Tumor Necrosis Factor-alpha/genetics
- Skin/metabolism
- Molecular Sequence Data
- Flounder/genetics*
- Complement C3/genetics
- DNA Primers
- Myocardium/metabolism
- Base Sequence
- Keratins/genetics
- Animals, Genetically Modified/genetics
- Animals
- Sequence Analysis, DNA
- PubMed
- 16027989 Full text @ Mar. Biotechnol.
Citation
Yazawa, R., Hirono, I., and Aoki, T. (2005) Characterization of Promoter Activities of Four Different Japanese Flounder Promoters in Transgenic Zebrafish. Marine biotechnology (New York, N.Y.). 7(6):625-633.
Abstract
An important consideration in transgenic research is the choice of promoter for regulating the expression of a foreign gene. In this study several tissue-specific and inducible promoters derived from Japanese flounder Paralichthys olivaceus were identified, and their promoter activity was examined in transgenic zebrafish. The 5' flanking regions of the Japanese flounder complement component C3, gelatinase B, keratin, and tumor necrosis factor (TNF) genes were linked to green fluorescence protein (GFP) as a reporter gene. The promoter regulatory constructs were introduced into fertilized zebrafish eggs. As a result we obtained several stable transgenic zebrafish that displayed green fluorescence in different tissues. Complement component C3 promoter regulated GFP expression in liver, and gelatinase B promoter regulated it in the pectoral fin and gills. Keratin promoter regulated GFP expression in skin and liver. TNF gene promoter regulated GFP expression in the pharynx and heart. TNF promoter had lipoplysaccharide-inducible activity, such that when transgenic embryos were immersed lipopolysaccharide, GFP expression increased in the epithelial tissues. These 4 promoters regulated the expression of GFP in different patterns in transgenic zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping