PUBLICATION
New ways to admire zebrafish: progress in functional genomics research methodology
- Authors
- Teh, C., Parinov, S., and Korzh, V.
- ID
- ZDB-PUB-050718-6
- Date
- 2005
- Source
- Biotechniques 38(6): 897-906 (Review)
- Registered Authors
- Korzh, Vladimir, Parinov, Serguei, Teh, Cathleen
- Keywords
- none
- MeSH Terms
-
- Animals
- Cloning, Molecular
- DNA Transposable Elements
- Electroporation
- Genomics*
- HSP70 Heat-Shock Proteins/genetics
- Male
- Mutagenesis
- Research
- Spermatozoa/ultrastructure
- Transgenes
- Zebrafish/genetics*
- PubMed
- 16018551 Full text @ Biotechniques
Citation
Teh, C., Parinov, S., and Korzh, V. (2005) New ways to admire zebrafish: progress in functional genomics research methodology. Biotechniques. 38(6):897-906.
Abstract
The main challenge of the post-genomic era is to functionally characterize genes identified by the genome sequencing projects. Model organisms, including zebrafish, are indispensable for this demanding task. Zebrafish has been successfully incorporated into large-scale genetic screens due to the optical clarity of the embryos and their accessibility to various experimental techniques throughout development. The attractiveness of the zebrafish as a model organism is enhanced by the availability of continuously improving genomic tools and methodologies for functional characterization of the gene. This article will highlight the current techniques used in the field, with the focus on transgenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping