PUBLICATION
Essential roles of G{alpha}12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements
- Authors
- Lin, F., Sepich, D.S., Chen, S., Topczewski, J., Yin, C., Solnica-Krezel, L., and Hamm, H.
- ID
- ZDB-PUB-050607-6
- Date
- 2005
- Source
- The Journal of cell biology 169(5): 777-787 (Journal)
- Registered Authors
- Lin, Fang, Sepich, Diane, Solnica-Krezel, Lilianna, Topczewski, Jacek, Yin, Chunyue
- Keywords
- none
- MeSH Terms
-
- Molecular Sequence Data
- Gene Expression Regulation, Developmental/physiology
- Signal Transduction/physiology
- Cell Shape/physiology
- GTP-Binding Protein alpha Subunits, G12-G13/genetics
- GTP-Binding Protein alpha Subunits, G12-G13/metabolism*
- Embryonic Development/physiology*
- Wnt Proteins
- Gastrula/cytology
- Gastrula/metabolism*
- Intercellular Signaling Peptides and Proteins/metabolism
- Body Patterning/physiology
- Humans
- Cell Communication/physiology
- Receptors, G-Protein-Coupled/genetics
- Receptors, G-Protein-Coupled/metabolism
- Mesoderm/cytology
- Mesoderm/metabolism*
- Cell Line
- Animals
- Cell Movement/physiology
- Zebrafish/embryology*
- Zebrafish/metabolism*
- PubMed
- 15928205 Full text @ J. Cell Biol.
Citation
Lin, F., Sepich, D.S., Chen, S., Topczewski, J., Yin, C., Solnica-Krezel, L., and Hamm, H. (2005) Essential roles of G{alpha}12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements. The Journal of cell biology. 169(5):777-787.
Abstract
Galpha(12/13) have been implicated in numerous cellular processes, however, their roles in vertebrate gastrulation are largely unknown. Here, we show that during zebrafish gastrulation, suppression of both Galpha(12) and Galpha(13) signaling by overexpressing dominant negative proteins and application of antisense morpholino-modified oligonucleotide translation interference disrupted convergence and extension without changing embryonic patterning. Analyses of mesodermal cell behaviors revealed that Galpha(12/13) are required for cell elongation and efficient dorsalward migration during convergence independent of noncanonical Wnt signaling. Furthermore, Galpha(12/13) function cell-autonomously to mediate mediolateral cell elongation underlying intercalation during notochord extension, likely acting in parallel to noncanonical Wnt signaling. These findings provide the first evidence that Galpha(12) and Galpha(13) have overlapping and essential roles in distinct cell behaviors that drive vertebrate gastrulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping