PUBLICATION
bloodthirsty, an RBCC/TRIM gene required for erythropoiesis in zebrafish
- Authors
- Yergeau, D.A., Cornell, C.N., Parker, S.K., Zhou, Y., and Detrich, H.W. 3rd.
- ID
- ZDB-PUB-050518-3
- Date
- 2005
- Source
- Developmental Biology 283(1): 97-112 (Journal)
- Registered Authors
- Detrich, H. William, Zhou, Yi
- Keywords
- Chaenocephalus aceratus; Notothenia coriiceps; Danio rerio; B30.2; bloodthirsty (bty) gene; Icefish; Rockcod; Zebrafish; Erythropoiesis; Hematopoiesis; RING-B box-coiled-coil (RBCC); Tripartite motif (TRIM)
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Carrier Proteins/chemistry
- Carrier Proteins/genetics*
- Cell Differentiation
- DNA Primers
- DNA, Complementary/genetics
- Embryo, Nonmammalian/physiology*
- Erythropoiesis/physiology*
- Gene Expression Regulation, Developmental*
- In Situ Hybridization
- Molecular Sequence Data
- Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Sequence Homology, Amino Acid
- Species Specificity
- Transcription, Genetic
- Zebrafish/embryology*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- PubMed
- 15890331 Full text @ Dev. Biol.
Citation
Yergeau, D.A., Cornell, C.N., Parker, S.K., Zhou, Y., and Detrich, H.W. 3rd. (2005) bloodthirsty, an RBCC/TRIM gene required for erythropoiesis in zebrafish. Developmental Biology. 283(1):97-112.
Abstract
The Antarctic icefishes (family Channichthyidae, suborder Notothenioidei) constitute the only vertebrate taxon that fails to produce red blood cells. These fishes can be paired with closely related, but erythrocyte-producing, notothenioids to discover erythropoietic genes via representational difference analysis. Using a B30.2-domain-encoding DNA probe so derived from the hematopoietic kidney (pronephros) of a red-blooded Antarctic rockcod, Notothenia coriiceps, we discovered a related, novel gene, bloodthirsty (bty), that encoded a 547-residue protein that contains sequential RING finger, B Box, coiled-coil, and B30.2 domains. bty mRNA was expressed by the pronephric kidney of N. coriiceps at a steady-state level 10-fold greater than that found in the kidney of the icefish Chaenocephalus aceratus. To test the function of bty, we cloned the orthologous zebrafish gene from a kidney cDNA library. Whole-mount in situ hybridization of zebrafish embryos showed that bty mRNA was present throughout development and, after the mid-blastula transition, was expressed in the head and in or near the site of primitive erythropoiesis in the tail just prior to red cell production. One- to four-cell embryos injected with two distinct antisense morpholino oligonucleotides (MOs) targeted to the 5'-end of the bty mRNA failed to develop red cells, whereas embryos injected with 4- and 5-bp mismatch control MOs produced wild-type quantities of erythrocytes. The morphant phenotype was rescued by co-injection of synthetic bty mRNA containing an artificial 5'-untranslated region (UTR) with the antisense MO that bound the 5'-UTR of the wild-type bty transcript. Furthermore, the expression of genes that mark terminal erythroid differentiation was greatly reduced in the antisense-MO-treated embryos. We conclude that bty is likely to play a role in differentiation of the committed red cell progenitor.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping