ZFIN ID: ZDB-PUB-050513-8
The isolation, characterization, and expression of a novel GDF11 gene and a second myostatin form in zebrafish, Danio rerio
Biga, P.R., Roberts, S.B., Iliev, D.B., McCauley, L.A., Moon, J.S., Collodi, P., and Goetz, F.W.
Date: 2005
Source: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology   141(2): 218-230 (Journal)
Registered Authors: Collodi, Paul, Goetz, Frederick W., Moon, Jesung
Keywords: GDF11; Zebrafish; Myostatin; TGF-?
MeSH Terms:
  • Animals
  • Base Sequence
  • Bone Morphogenetic Proteins/genetics*
  • Bone Morphogenetic Proteins/isolation & purification
  • Gene Components
  • Growth Differentiation Factors
  • Molecular Sequence Data
  • Myostatin
  • Phylogeny
  • RNA, Messenger/analysis
  • Sequence Alignment
  • Tissue Distribution
  • Transforming Growth Factor beta/genetics*
  • Transforming Growth Factor beta/isolation & purification
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/isolation & purification
PubMed: 15886039 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
In the current study, the first non-mammalian growth/differentiation factor (GDF) 11-like homolog was cloned from zebrafish. At the nucleotide level, zebrafish GDF11 is most similar to human GDF11 (79%), while the peptide is most similar to mouse GDF11 (78%). Phylogenetic analysis showed that the zebrafish GDF11 clusters with mammalian GDF11s. This study also cloned a second MSTN form in zebrafish most similar to Salmonid MSTN2 forms. Based on real time PCR, GDF11 is expressed in multiple adult tissues, with levels highest in whole heads and gonads, and expression is less ubiquitous when compared to MSTN expression. During embryonic development, real time PCR demonstrated increasing GDF11 mRNA levels 10 h post-fertilization (hpf), while MSTN mRNA levels remain low until 48 hpf. This is the first report of a transforming growth factor (TGF)-beta superfamily member in a non-mammalian species that is more closely related to GDF11 than MSTN, and also a second form of MSTN in zebrafish; suggesting that a more complex TGF-beta superfamily array exists in primitive vertebrates than previously thought.