PUBLICATION

Changes in glutamate receptor subunit 4 expression in the deafferented olfactory bulb of zebrafish

Authors
Fuller, C.L., Villanueva, R., and Byrd, C.A.
ID
ZDB-PUB-050513-7
Date
2005
Source
Brain research   1044(2): 251-261 (Journal)
Registered Authors
Byrd-Jacobs, Christine
Keywords
iGluR4; AMPA receptor; Zebrafish; Olfactory bulb; Deafferentation; Periglomerular cells
MeSH Terms
  • Animals
  • Blotting, Western
  • Cell Count
  • Denervation/adverse effects
  • Female
  • Gene Expression Regulation/physiology*
  • Immunohistochemistry/methods
  • Male
  • Neurons/metabolism*
  • Olfactory Bulb/cytology*
  • Olfactory Bulb/physiology
  • Receptors, AMPA/metabolism*
  • Time Factors
  • Tyrosine 3-Monooxygenase/metabolism
  • Zebrafish
PubMed
15885223 Full text @ Brain Res.
Abstract
The distribution of ionotropic glutamate receptor subunit 4 (iGluR4) was examined in both normal and deafferented olfactory bulbs of adult zebrafish, Danio rerio. With the exception of the olfactory nerve layer, there was extensive labeling with antibodies to iGluR4 in the olfactory bulbs, specifically in juxtaglomerular cell bodies and their processes. These results are consistent with previous work, which has suggested differential distribution of glutamate receptors in the vertebrate olfactory system. Analysis of bulbs following olfactory organ removal revealed a significant loss of iGluR4 immunoreactivity by 24 h post-deafferentation. At 48 h after denervation, iGluR4 labeling had returned to normal levels and was retained through 3 weeks post-surgery. Thus, afferent input plays a role in reduced labeling of this protein immediately following injury, but return of immunoreactivity can occur even without sensory innervation.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping