PUBLICATION

Zebrafish as a model for caveolin-associated muscle disease; caveolin-3 is required for myofibril organization and muscle cell patterning

Authors
Nixon, S.J., Wegner, J., Ferguson, C., Méry, P.F., Hancock, J.F., Currie, P.D., Key, B., Westerfield, M., and Parton, R.G.
ID
ZDB-PUB-050513-13
Date
2005
Source
Human molecular genetics   14(13): 1727-1743 (Journal)
Registered Authors
Currie, Peter D., Key, Brian, Wegner, Jeremy, Westerfield, Monte
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Caveolin 3
  • Caveolins/genetics
  • Caveolins/metabolism*
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • Cell Fusion
  • Embryo, Nonmammalian/embryology
  • Humans
  • Molecular Sequence Data
  • Muscle, Skeletal/embryology*
  • Muscle, Skeletal/ultrastructure
  • Myoblasts, Skeletal/metabolism*
  • Myoblasts, Skeletal/ultrastructure
  • Myofibrils/genetics
  • Myofibrils/metabolism*
  • Myofibrils/ultrastructure
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
15888488 Full text @ Hum. Mol. Genet.
Abstract
Caveolae are an abundant feature of many animal cells. However, the exact function of caveolae remains unclear. We have used the zebrafish, Danio rerio, as a system to understand caveolae function focusing on the muscle-specific caveolar protein, caveolin-3 (Cav3). We have identified caveolin-1 (alpha and beta), caveolin-2 and caveolin-3 in the zebrafish. Zebrafish Cav3 has 72% identity to human CAV3, and the amino acids altered in human muscle diseases are conserved in the zebrafish protein. During embryonic development, cav3 expression is apparent by early segmentation stages in the first differentiating muscle precursors, the adaxial cells, and slightly later in the notochord. cav3 expression appears in the somites during mid-segmentation stages and then later in the pectoral fins and facial muscles. Cav3 and caveolae are located along the entire sarcolemma of late stage embryonic muscle fibers whereas beta-dystroglycan is restricted to the muscle fiber ends. Down-regulation of Cav3 expression causes gross muscle abnormalities and uncoordinated movement. Ultrastructural analysis of isolated muscle fibers reveals defects in myoblast fusion, and disorganized myofibril and membrane systems. Expression of the zebrafish equivalent of a human muscular dystrophy mutant, CAV3P104L, causes severe disruption of muscle differentiation. In addition, knock-down of caveolin-3 resulted in a dramatic up-regulation of eng1a expression resulting in an increase in the number of muscle pioneer-like cells adjacent to the notochord. These studies provide new insights into the role of Cav3 in muscle development and demonstrate its requirement for correct intracellular organization and myoblast fusion.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes